Genetic characterization of the M RNA segment of a Balkan Crimean-Congo hemorrhagic fever virus strain
Genetic characterization of the M RNA segment of a Balkan Crimean-Congo hemorrhagic fever virus strain
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DOI：
10.1002/jmv.20290
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发表时间：
2005-03-01
影响因子：
12.7
通讯作者：
Antoniadis, A
中科院分区：
文献类型：
--
作者：
Papa, A;Papadimitriou, E;Antoniadis, A
克里米亚-刚果出血热（CCHF）病毒是人类最严重的疾病之一，死亡率高达30%。它通过硬蜱叮咬或接触人类患者或受感染牲畜的血液或组织传播给人类。巴尔干半岛的半岛是该疾病的流行地区，每年都会观察到散发病例甚至暴发。M RNA片段编码两种表面糖蛋白Gn和Gc的糖蛋白前体。到目前为止，已公布了从尼日利亚、中国、巴基斯坦、塔吉克斯坦和俄罗斯分离的毒株的完整M RNA CCHF病毒序列。本研究报道了2001年夏季从科索沃的一个人死亡病例中分离的巴尔干半岛的CCHF病毒株Kosovo/9553/2001的M RNA片段的全核苷酸序列的遗传特征。这是首次公布的巴尔干半岛分离的CCHF病毒株的完整M核苷酸序列。发现巴尔干半岛的毒株与俄罗斯毒株相似，这两种毒株在核苷酸水平上与所有其他完全测序的CCHF病毒毒株的差异约为22%，在系统发育树中形成了一个独立的分支。(C)2005 Wiley-Liss，Inc.
Crimean-Congo hemorrhagic fever (CCHF) virus causes one of the most severe diseases in humans, with a mortality rate of up to 30%. It is transmitted to humans by the bite of hard ticks or by contact with blood or tissues from human patients or infected livestock. Balkan Peninsula is an endemic region of the disease, and sporadic cases or even outbreaks are observed every year. The M RNA segment encodes for the glycoprotein precursor of two surface glycoproteins Gn and Gc. Up to now complete M RNA CCHF virus sequences have been published from strains isolated in Nigeria, China, Pakistan, Tajikistan, and Russia. In the present study, the genetic characterization of the complete nucleotide sequence of the M RNA segment of a Balkan CCHF virus strain, Kosovo/9553/2001, isolated in summer of 2001 from a human fatal case in Kosovo is reported. This is the first published complete M nucleotide sequence of a CCHF virus strain isolated in Balkans. It was found that the Balkan strain is similar to the Russian strain, both strains differing from all other completely sequenced CCHF virus strains by approximately 22% at the nucleotide level forming an independent clade in the phylogenetic tree. (C) 2005 Wiley-Liss, Inc.