Studies on proteins in post-ecdysial nymphal cuticle of locust, Locusta migratoria, and cockroach, Blaberus craniifer
Studies on proteins in post-ecdysial nymphal cuticle of locust, Locusta migratoria, and cockroach, Blaberus craniifer
复制标题
DOI：
10.1016/s0965-1748(00)00029-1
复制
发表时间：
2000-07-01
影响因子：
3.8
通讯作者：
Andersen, SO
中科院分区：
文献类型：
--
作者：
Andersen, SO
从蝗虫、蝗虫和蟑螂中龄若虫的角质层中提取蛋白质。从蝗虫提取物和蟑螂提取物中分离纯化了7种蛋白质和5种蛋白质，并测定了它们的氨基酸序列。聚丙烯酰胺凝胶电泳表明，这些蛋白质只存在于若虫蜕皮后沉积的层中。其中一种蝗虫若虫蛋白和一种蟑螂若虫蛋白含有68个残基，即RR-2序列，已有报道在其他昆虫的固体角质层中发现了几种蛋白质。其中两种蟑螂蛋白含有一个75个残基的基序，该基序也存在于甲虫幼虫/蛹角质层的蛋白质中，以及龙虾Homarus americanus和蜘蛛蜘蛛的外骨骼蛋白质中。该基序包含Rebers-Riddiford共识序列的一个变体，称为RR-3基序。其中一种蝗虫和三种蟑螂蜕皮后蛋白含有一个或多个18个残基的拷贝，以前在家蚕蛹角质层的蛋白质中报道过。这两个物种的若虫蜕皮后蛋白与注定要被硬化的角质层中的蜕皮前蛋白(磷酸盐蛋白)具有共同的特征；它们与成虫的蜕皮后角质层蛋白或来自柔软、柔韧的角质层的蛋白几乎没有相似之处。蜕皮后角质层蛋白的可能作用被讨论与已报道的结构有关。(C)2000爱思唯尔科学有限公司。保留所有权利。
Proteins were extracted from the cuticle of mid-instar nymphs of locusts, Locusta migratoria, and cockroaches, Blaberus craniifer. Seven proteins were purified from the locust extract and five from the cockroach extract, and their amino acid sequences were determined. Polyacrylamide gel electrophoresis indicates that the proteins are present only in the post-ecdysially deposited layer of the nymphal cuticles. One of the locust and one of the cockroach nymphal proteins contain a 68-residue motif, the RR-2 sequence, which has been reported for several proteins from the solid cuticles of other insect species. Two of the cockroach proteins contain a 75-residue motif, which is also present in a protein from the larval/pupal cuticle of a beetle, Tenebrio molitor, and in proteins from the exoskeletons of a lobster, Homarus americanus, and a spider, Araneus diadematus. The motif contains a variant of the Rebers-Riddiford consensus sequence, and is called the RR-3 motif. One of the locust and three of the cockroach post-ecdysial proteins contain one or more copies of an 18-residue motif, previously reported in a protein from Bombyx mori pupal cuticle. The nymphal post-ecdysial proteins from both species have features in common with pre-ecdysial proteins (pharate proteins) in cuticles destined to be sclerotised; they show little similarity to the post-ecdysial cuticular proteins from adult locusts or to proteins from soft, pliable cuticles. Possible roles for post-ecdysial cuticular proteins are discussed in relation to the reported structures. (C) 2000 Elsevier Science Ltd. All rights reserved.