Characterization of starvation-induced dispersion in Pseudomonas putida biofilms
Characterization of starvation-induced dispersion in Pseudomonas putida biofilms
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DOI：
10.1111/j.1462-2920.2005.00775.x
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发表时间：
2005-06-01
影响因子：
5.1
通讯作者：
Tolker-Nielsen, T
中科院分区：
文献类型：
--
作者：
Gjermansen, M;Ragas, P;Tolker-Nielsen, T
生物膜生活方式，微生物细胞聚集的地方，因为细胞间的相互连接的化合物的表达，被认为是对环境中的微生物至关重要。由于微生物必须能够在无根状态和浮游状态之间交替，因此预计它们必须能够调节其形成生物膜和溶解生物膜的能力。我们提出了一个生物膜溶解过程的调查发生在流动室生长的恶臭假单胞菌生物膜。局部饥饿诱导的生物膜溶解似乎是恶臭假单胞菌生物膜发育的一个组成部分，引起特征性的结构重排。整个恶臭假单胞菌生物膜的快速全球溶解被证明是对碳饥饿的反应。遗传分析表明，邻近的恶臭假单胞菌基因PP0164和PP0165在恶臭假单胞菌生物膜的形成和溶解中起作用。PP0164编码一种已知的功能未知的质周蛋白，并且PP0164突变菌具有粘性，在碳饥饿反应中无法降低其粘性并溶解其生物膜。PP0165编码一种假定的含有GGDEF和EAL结构域的跨膜蛋白，并且PP0165突变菌无法增加其粘附性并形成生物膜。我们认为PP0164和PP0165蛋白参与了细菌粘附性的调控；PP0165蛋白通过c-di-GMP信号传导，而PP0164蛋白作为信号的换能器。
The biofilm lifestyle, where microbial cells are aggregated because of expression of cell-to-cell interconnecting compounds, is believed to be of paramount importance to microbes in the environment. Because microbes must be able to alternate between sessile and planktonic states, it is anticipated that they must be able to regulate their ability to form biofilm and to dissolve biofilm. We present an investigation of a biofilm dissolution process occurring in flow-chamber-grown Pseudomonas putida biofilms. Local starvation-induced biofilm dissolution appears to be an integrated part of P. putida biofilm development that causes characteristic structural rearrangements. Rapid global dissolution of entire P. putida biofilms was shown to occur in response to carbon starvation. Genetic analysis suggested that the adjacent P. putida genes PP0164 and PP0165 play a role in P. putida biofilm formation and dissolution. PP0164 encodes a putative periplasmic protein of previously unknown function, and PP0164 mutant bacteria are sticky, and unable to reduce their adhesiveness and dissolve their biofilm in response to carbon starvation. PP0165 encodes a putative transmembrane protein containing GGDEF and EAL domains, and PP0165 mutant bacteria are unable to increase their adhesiveness and form biofilm. We suggest that the PP0164 and PP0165 proteins are involved in the regulation of the adhesiveness of the bacteria; the PP0165 protein through c-di-GMP signalling, and the PP0164 protein as a transducer of the signal.