Inhibitory potential of EGCG on Streptococcus mutans biofilm: A new approach to prevent Cariogenesis
Inhibitory potential of EGCG on Streptococcus mutans biofilm: A new approach to prevent Cariogenesis
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DOI：
10.1016/j.micpath.2020.104129
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发表时间：
2020-06-01
影响因子：
3.8
通讯作者：
Thirugnanasambantham, Krishnaraj
中科院分区：
文献类型：
--
作者：
Islam, Mohamed Ibrahim Hairul;Arokiyaraj, Selvaraj;Thirugnanasambantham, Krishnaraj
龋齿是牙齿脱落的常见原因，变形链球菌被确定为致病病原体。研究了表没食子儿茶素没食子酸酯（EGCG）对变形链球菌葡聚糖酶及其生物膜的抑制作用。使用AutoDock工具和酶抑制实验验证葡聚糖酶结合和EGCG的抑制潜力。用扫描电镜（SEM）分析了人类牙齿样品的生物膜抑制电位。分子对接发现，EGCG与GLU 515和TRP 517氨基酸相互作用，并与葡聚糖酶结合。扫描电镜分析显示，不同浓度的EGCG对牙齿样品表面的变形链球菌生物膜有抑制作用。生物信息学和生物学分析证实，EGCG可能与变形链球菌葡聚糖酶结合并抑制其酶活性。酶抑制葡聚糖酶可减弱变形链球菌在牙表面形成生物膜的潜力。因此，我们认为牙齿表面变形链球菌生物膜的EGCG抑制潜力是预防龋齿的新途径。
Dental caries is a common cause for tooth loss and Streptococcus mutans is identified as the etiologic pathogen. This study evaluates the inhibitory potential of Epigallocatechin gallate (EGCG) on S.mutans glucansucrase enzyme and its biofilm. Glucansucrase binding and the inhibitory potential of EGCG was validated using AutoDock tool and enzyme inhibitory assay. Biofilm inhibitory potential was also confirmed using Scanning Electron Microscopic (SEM) analysis in human tooth samples. Molecular docking revealed that EGCG interacted with GLU 515 and TRP 517 amino acids and binds to glucansucrase. SEM analysis revealed inhibition of S.mutans biofilm by various concentrations of EGCG on surfaces of tooth samples. Bioinformatics and biological assays confirmed that EGCG potentially binds to the S. mutans glucansucrase and inhibits its enzymatic activity. Enzymatic inhibition of glucansucrase attenuated biofilm formation potential of S. mutans on tooth surface. Thus, we conclude that EGCG inhibitory potential of S. mutans biofilm on the tooth surface is a novel approach in prevention of dental caries.