Picosecond kinetics of the initial photochemical electron-transfer reaction in bacterial photosynthetic reaction centers.
Picosecond kinetics of the initial photochemical electron-transfer reaction in bacterial photosynthetic reaction centers.
复制标题
细菌光合反应中心初始光化学电子转移反应的皮秒动力学。
DOI：
10.1021/bi00347a002
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发表时间：
1985
期刊：
影响因子：
2.9
通讯作者：
Parson,WW
中科院分区：
文献类型：
--
作者：
Woodbury,NW;Becker,M;Middendorf,D;Parson,WW
材料与方法反应中心由Rps制备。sphaeroides如前所述（Schenck等，1982），并悬浮于0.1%月桂基二甲基氧化胺（LDAO）和0.01M三（羟甲基）氨基甲烷盐酸盐（Tris-HCl），pH 8.0中，用于在295 K下测量。对于一些低温测量，添加56%（v/v）甘油。对于大多数低温测量，基本上如所述将反应中心置于聚乙烯醇（PVA）膜中
Materials and MethodsReaction centers were prepared from Rps. sphaeroides as described previously (Schenck et al., 1982) and were suspended in 0.1% lauryldimethylamine oxide (LDAO) and 0.01 M tris (hydroxymethyl) aminomethane hydrochloride (Tris-HCl), pH 8.0, for measurements at 295 K. For some low-temper-ature measurements, 56%(v/v) glycerol was added. For most low-temperature measurements, reactioncenters were placed in polyvinyl alcohol (PVA) films essentially as described