Effect of smoking on the serum levels of 25-hydroxyvitamin D depends on the assay employed
Effect of smoking on the serum levels of 25-hydroxyvitamin D depends on the assay employed
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DOI：
10.1530/eje-10-0150
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发表时间：
2010-08-01
影响因子：
5.8
通讯作者：
Jorde, Rolf
中科院分区：
文献类型：
--
作者：
Grimnes, Guri;Almaas, Bjorg;Jorde, Rolf
目的：因为我们发现吸烟者的血清25-羟基维生素D（25（OH）D）水平高于非吸烟者，使用Roche的电化学发光免疫测定法（ECLIA）进行分析，本研究的目的是检查吸烟者和非吸烟者之间的这种差异是否使用其他血清25（OH）D测定法维持。设计：对1994-1995年Tromso研究的6932名参与者进行了一项基于人群的横断面研究，并对53名非吸烟者和54名吸烟者进行了一项比较6种不同血清25（OH）D测定的验证研究。在基于人群的研究中，使用具有多变量调整的一般线性模型评估了吸烟、季节和由ECLIA（Roche）测量的血清25（OH）D之间的关联。在验证研究中，使用来自两个不同实验室（RIA（DiaSorin）、HPLC、RIA（IDS）和ECLIA（Roche））的液相色谱-质谱联用分析法分析了血清25（OH）D水平。结果：在以人群为基础的研究中，ECLIA法测定的吸烟者、既往吸烟者和现吸烟者血清25（OH）D水平分别为51.9、53.2和72.0nmol/l（P
Objective: Because we found higher serum 25-hydroxyvitamin D (25(OH) D) levels among smokers than among non-smokers with analyses using an electrochemiluminescence immunoassay (ECLIA) from Roche, the purpose of the present study was to examine whether this difference between smokers and non-smokers was maintained using other serum 25(OH) D assays.Design: A cross-sectional population-based study on 6932 participants from the Tromso study, 1994-1995, and one validation study comparing six different serum 25(OH) D assays in 53 non-smokers and 54 smokers were performed.Methods: The association between smoking, season and serum 25(OH) D as measured by ECLIA (Roche) was assessed in the population-based study using general linear models with multivariate adjustments. In the validation study, serum levels of 25(OH) D were analysed with liquid chromatography coupled with mass spectrometry assay from two different laboratories, RIA (DiaSorin), HPLC, RIA (IDS) and ECLIA (Roche). T-tests and linear mixed model analyses were performed to compare the serum 25(OH) D levels in smokers and non-smokers within and between the methods.Results: In the population-based study, the serum levels of 25(OH) D using the ECLIA method were 51.9, 53.2 and 72.0 nmol/l in never, former and current smokers (P