The role of organic volatile profiles in clinical diagnosis.
The role of organic volatile profiles in clinical diagnosis.
复制标题
有机挥发物特征在临床诊断中的作用。
作者：
A. Zlatkis;R. Brazell;C. F. Poole
生物体液中的有机挥发性成分包含了临床上有用的诊断信息，用于识别人体代谢紊乱。为了获得这些信息，有必要开发可重复地从生物体液中提取痕量挥发性成分的方法（动态顶空、气相汽提、溶剂萃取和蒸发器技术），通过高分辨率毛细管柱气相色谱法分离复杂的提取物，并开发计算机辅助数据处理和模式识别技术，用于分析产生的大量信息。通过气相色谱-质谱法在尿液、血清和母乳中鉴定的正常和病理性有机挥发物列表。上述技术的研究和诊断糖尿病，呼吸道病毒感染，肾功能不全，和癌症的临床应用进行了说明。
The organic volatile constituents of biological fluids contain clinically useful diagnostic information for the recognition of metabolic disorders in man. To gain access to this information, it was necessary to develop the methodology for reproducibly stripping the trace concentrations of volatiles from biological fluids (dynamic headspace, gas phase-stripping, solvent extraction, and the transevaporator technique), to separate the complex extracts by high-resolution capillary column gas chromatography, and to develop computer-aided data-handling and pattern-recognition techniques for analyzing the immense amount of information generated. The normal and pathological organic volatiles identified by gas chromatography--mass spectrometry in urine, serum, and breast milk are tabulated. Clinical applications of the above techniques to the study and diagnosis of diabetes mellitus, respiratory virus infection, renal insufficiency, and cancer are described.