An efficient treatment of biofilm-induced periodontitis using Pt nanocluster catalysis
An efficient treatment of biofilm-induced periodontitis using Pt nanocluster catalysis
复制标题
利用 Pt 纳米簇催化有效治疗生物膜引起的牙周炎
作者：
Wu Tao;Sun Jiahui;Lei Jie;Fan Qihang;Tang Xiuyu;Zhu Guixin;Yan Qi;Feng Xiaobo;Shi Bin
牙周炎是一种常见的慢性炎症性疾病，与生物膜形成、牙龈退缩和支持性骨丢失相关，可导致牙周袋形成，最终导致牙齿脱落。目前临床上通过洁治和抗生素治疗牙周炎仍面临着不可避免的出血、牙周组织损伤、耐药性和治疗不足等问题。本文制备了一种具有催化活性的可注射的抗牙周炎软膏，该软膏由Pt纳米簇（PtNC）修饰的g-C3 N4（CN）和PEG 400/PEG 4000组成，其有效地治疗生物膜感染的牙周炎。采用温和的紫外光（UV）照射，在CN表面形成了含量极低（0.07%）的PtNCs（<2nm）。由于CN-PtNCs具有较强的O2吸附和活化能力以及它们之间的电子转移，它们在黑暗中同时表现出氧化酶和过氧化物酶的活性，并产生活性氧（ROS）。CN-PtNCs对金黄色葡萄球菌（S.金黄色葡萄球菌）和大肠杆菌（E. coli）。此外，得益于CN-PtNC良好的生物相容性和PEG 400/PEG 4000软膏的可注射性，CN-PtNC软膏具有高生物利用度，成功治疗大鼠牙周炎，减轻炎症和减少骨丢失，表现出优于派丽奥的性能。因此，这种催化系统是一种有效的，非侵入性的，和牙周炎的无菌治疗的前景。
Periodontitis is a common chronic inflammatory disease associated with biofilm formation, gingival recession, and supporting bone loss that can lead to the formation of periodontal pockets and, ultimately, tooth loss. Clinical treatment for periodontitis through scaling and antibiotics still faces the problems of unavoidable bleeding, injury to periodontal tissue, drug resistance, and insufficient treatment. Herein we prepared an injectable anti-periodontitis ointment with catalytic activity that consists of Pt nanocluster (PtNC) modified g-C3N4 (CN), and PEG400/PEG4000, which efficiently treated biofilm-infected periodontitis. PtNCs (<2 nm) with ultralow content (0.07%) were formed on the surface of CN using mild ultraviolet (UV) irradiation. Due to the strong O2 adsorption and activation ability of CN-PtNCs and their mutual electron transfer, they show both oxidase-like and peroxidase-like activities and produce reactive oxygen species (ROS) in the dark. CN-PtNCs showed strong biofilm elimination ability towards Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). Furthermore, benefiting from the good biocompatibility of CN-PtNCs and the injectable property of the PEG400/PEG4000 ointment, the CN-PtNC ointment with high bioavailability successfully treated periodontitis in rats, alleviating inflammation and reducing bone loss, and showed better performance than periocline. Therefore, this catalytic system is promising for an efficient, non-invasive, and antibiotic-free treatment of periodontitis.