Effect of hemoglobin concentration on maximal O2 uptake in canine gastrocnemius muscle in situ.
Effect of hemoglobin concentration on maximal O2 uptake in canine gastrocnemius muscle in situ.
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血红蛋白浓度对犬腓肠肌原位最大摄氧量的影响。
DOI：
10.1152/jappl.1991.70.3.1105
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Wagner,PD
中科院分区：
文献类型：
--
作者：
Hogan,MC;Bebout,DE;Wagner,PD
通过改变血红蛋白（Hb）浓度和动脉血氧分压（PaO 2）来降低最大工作肌的O2输送量，以研究[Hb]降低时最大摄氧量（VO 2 max）的降低是否与有效肌肉O2弥散量（DmO 2）的变化部分相关。进行了两组实验。在初始组（n = 8）中，以随机顺序使用血液中的三个Hb水平[5.8 +/- 0.3、9.4 +/- 0.1和14.4 +/- 0.6（SE）g/100 ml]，以相等的肌肉血流量和PaO 2最大工作离体犬腓肠肌进行泵灌注。VO 2 max随[Hb]的降低而降低，但VO 2 max与流出静脉PO 2（PvO 2）和计算的平均毛细血管PO 2（PcO 2）之间的关系在原点上不是线性的，因此，与DmO 2的单个值不相容（使用基于菲克扩散定律的模型通过玻尔积分计算）。为了澄清这些结果，进行第二组实验（n = 6），其中两个水平的Hb（14.0 +/- 0.6和6.9 +/- 0.6 g/100 ml）分别与两个氧合水平相结合（PaO 2 79 +/- 8和29 +/- 2 Torr），并以随机顺序施加，以恒定血流再次泵送灌注最大工作犬腓肠肌。在这些实验中，VO 2 max与每种[Hb]的PvO 2和计算的PcO 2之间的关系与当PaO 2降低时DmO 2的恒定估计值一致，但较低[Hb]的计算的DmO 2比较高[Hb]的低33%（P <0.05）。（250字处删节）
O2 delivery to maximally working muscle was decreased by altering hemoglobin (Hb) concentration and arterial PO2 (PaO2) to investigate whether the reductions in maximal O2 uptake (VO2max) that occur with lowered [Hb] are in part related to changes in the effective muscle O2 diffusing capacity (DmO2). Two sets of experiments were conducted. In the initial set (n = 8), three levels of Hb [5.8 +/- 0.3, 9.4 +/- 0.1, and 14.4 +/- 0.6 (SE) g/100 ml] in the blood were used in random order to pump perfuse, at equal muscle blood flows and PaO2, maximally working isolated dog gastrocnemius muscle. VO2max declined with decreasing [Hb], but the relationship between VO2max and both the effluent venous PO2 (PvO2) and the calculated mean capillary PO2 (PcO2) was not linear through the origin and, therefore, not compatible with a single value of DmO2 (as calculated by Bohr integration using a model based on Fick's law of diffusion). To clarify these results, a second set of experiments (n = 6) was conducted in which two levels of Hb (14.0 +/- 0.6 and 6.9 +/- 0.6 g/100 ml) were each combined with two levels of oxygenation (PaO2 79 +/- 8 and 29 +/- 2 Torr) and applied in random sequence to again pump perfuse maximally working dog gastrocnemius muscle at constant blood flow. In these experiments, the relationship between VO2max and both PvO2 and calculated PcO2 for each [Hb] was consistent with a constant estimate of DmO2 as PaO2 was reduced, but the calculated DmO2 for the lower [Hb] was 33% less than that at the higher [Hb] (P less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)