Fermentation characteristics and probiotic activity of a purified fraction of polysaccharides from Fuzhuan brick tea
Fermentation characteristics and probiotic activity of a purified fraction of polysaccharides from Fuzhuan brick tea
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DOI：
10.1016/j.fshw.2021.12.030
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发表时间：
2022-05-01
影响因子：
7
通讯作者：
Liu, Zhonghua
中科院分区：
文献类型：
--
作者：
Chen, Guijie;Zeng, Ziqi;Liu, Zhonghua
茯砖茶多糖是茶叶中最重要的生物活性成分之一，具有多种保健功能。我们以前的工作表明，粗FBTPS（CFBTPS）可以调节肠道菌群。然而，CFBTPS中哪种纯化组分有助于调节肠道微生物群仍不清楚。因此，在这项工作中，CFBTPS的纯化级分（FBTPS-2-1）的发酵特性和益生菌活性进行了评价。结果表明，肠道菌群可以利用FBTPS-2-1产生短链脂肪酸，包括乙酸、丙酸、正丁酸和正戊酸。FBTPS-2-1可以调节肠道菌群的结构和代谢途径。FBTPS-2-1可增加肠道菌群中的普雷沃氏菌科和双歧杆菌科等有益菌群，减少肠道菌科和梭杆菌科等有害菌群。宏基因组学分析结果表明，在FBTPS-2-1发酵后，优势菌为普雷沃氏菌（Prevotella copri）和巨型单胞菌（Megamonas funiformis）。此外，FBTPS-2-1还可以调节肠道菌群的生物合成和代谢途径。因此，由于肠道微生物群的调节，预计用FBTPS-2-1富集食物是促进人类健康的潜在策略。(c)2022北京市食品科学研究院。Elsevier B. V.代表KeAi Communications Co.提供出版服务，这是一个在CC BY-NC-ND许可证下的开放获取文章（http：//creativecommons.org/licenses/by-nc-nd/4.0/）。
Polysaccharides from Fuzhuan brick tea (FBTPS), one of most important bioactive components in tea, showed various health-promoting functions. Our previous work demonstrated that the crude FBTPS (CFBTPS) could modulate the gut microbiota. However, which purified fraction in CFBTPS contributing to the modulation of gut microbiota remains unclear. Thus, the fermentation characteristics and probiotic activity of a purified fraction (FBTPS-2-1) of CFBTPS were evaluated in this work. The results showed that gut microbiota could utilize FBTPS-2-1 to produce short-chain fatty acids including acetic, propionic, n-butyric and n-valeric acids. FBTPS-2-1 could modulate the structure and metabolic pathways of gut microbiota. FBTPS-2-1 could increase the health-promoting gut microbiota such as Prevotellaceae and Bifidobacteriaceae, and decreased the harmful bacteria such as Enterobacteriaceae and Fusobacteriaceae. The results of metagenomics showed that Prevotella copri and Megamonas funiformis were the dominant bacteria after fermentation of FBTPS-2-1. Furthermore, FBTPS-2-1 could regulate the biosynthesis and metabolism pathways of gut microbiota. Thus, the enrichment of food with FBTPS-2-1 is expected as a potential strategy for promoting human health due to modulation of gut microbiota. (c) 2022 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).