NADPH oxidase involvement in cellular integrity
NADPH oxidase involvement in cellular integrity
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DOI：
10.1007/s00425-008-0716-2
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发表时间：
2008-05-01
期刊：
影响因子：
4.3
通讯作者：
Davies, Julia M.
中科院分区：
文献类型：
--
作者：
Macpherson, Neil;Takeda, Seiji;Davies, Julia M.
NADPH氧化酶的活性参与植物的适应和发育。来源于NADPH氧化酶活性的活性氧可能有助于壁强度和原生质体体积调节。NADPH氧化酶突变体rhd 2/Atrbohc的根毛隆起顶端比kjk纤维素合酶突变体更健壮，但比野生型（WT）更容易爆裂。根表皮壁出现受损rhd 2/Atrbohc，所揭示的壁降解酶释放的原生质体的数量。当用低渗低离子强度介质原位挑战时，rhd 2/Atrbohc的根毛凸起比WT爆裂更多。用二苯碘鎓抑制NADPH氧化酶活性导致WT表型复制响应于低渗休克的rhd 2/Atrbohc爆发。这暗示RHD 2/AtRBOHC软化细胞壁以允许原生质体扩增。总的来说，结果表明RHD 2/AtRBOHC在促进壁强度方面的作用。
NADPH oxidase activity is involved in plant adaptation and development. The reactive oxygen species sourced by NADPH oxidase activity may contribute to wall strength and protoplast volume adjustment. Root hair bulge apices of the NADPH oxidase mutant rhd2/Atrbohc were more robust than the kjk cellulose synthase mutant, but burst more readily than the wild type (WT). Root epidermal wall appeared impaired in rhd2/Atrbohc, as revealed by the number of protoplasts released by wall-degrading enzymes. Root hair bulges of rhd2/Atrbohc burst more than the WT when challenged in situ with hypo-osmotic low ionic strength medium. Inhibition of NADPH oxidase activity with diphenylene iodonium caused WT to phenocopy the rhd2/Atrbohc bursting in response to hypo-osmotic shock. This implicates RHD2/AtRBOHC in softening the cell wall to permit protoplast expansion. Overall, the results point to a role for RHD2/AtRBOHC in contributing to wall strength.