Diagnosis and sequence analysis of avian leukosis virus subgroup J isolated from Chinese Partridge Shank chickens
Diagnosis and sequence analysis of avian leukosis virus subgroup J isolated from Chinese Partridge Shank chickens
复制标题
DOI：
10.3382/ps/pev040
复制
发表时间：
2015-04-01
期刊：
影响因子：
4.4
通讯作者：
Cui, Zhizhong
中科院分区：
文献类型：
--
作者：
Dong, Xuan;Zhao, Peng;Cui, Zhizhong
通过剖检、组织病理学检查、抗体检测、病毒分离、免疫荧光检测和序列分析，确诊中国Paralyshank鸡感染J亚群禽白血病病毒（ALV-J）。在J群中同时发现骨髓细胞瘤、骨髓瘤和纤维肉瘤。序列分析表明，3株地方鸡ALV-J毒株与分离自白色肉用型鸡的ALV-J毒株gp 85和gp 37具有高度同源性。系统进化树分析表明，本研究分离的3株鸡源致病性禽流感病毒与中国黄鸡的参考毒株和美国和中国白色肉鸡的部分毒株亲缘关系较近。本研究首次发现J型ALV-J感染ParanumShank鸡，并在该批本地鸡中同时发现骨髓瘤、骨髓瘤和纤维肉瘤。
The diagnosis of avian leukosis virus subgroup J (ALV-J) infection in Chinese Partridge Shank chickens was confirmed by necropsy, histopathological examinations, antibody tests, viral isolation, immunofluorescence assays, and sequence analysis. Myelocytoma, myeloma, and fibrosarcoma were simultaneously found in Partridge Shank flock with ALV-J infection. Sequence analysis of the env genes of ALV-J demonstrated that both gp85 and gp37 were highly homologous among the three strains from local chickens of those among ALV-J strains isolated from white meat-type chickens. The phylogenetic trees indicated that the three strains isolated in this study were closely related to reference strains isolated in so-called Chinese yellow chickens and some strains isolated from white meat-type chickens, both from the USA and China. The observed ALV-J infection was the first report on Partridge Shank chickens, and myelocytoma, myeloma, and fibrosarcoma were found at the same time in this batch of local chickens.