Effects of aliphatic acids, furfural, and phenolic compounds on Debaryomyces hansenii CCMI 941
Effects of aliphatic acids, furfural, and phenolic compounds on Debaryomyces hansenii CCMI 941
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DOI：
10.1385/abab:121:1-3:0413
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发表时间：
2005-03-01
影响因子：
3
通讯作者：
Gírio, FM
中科院分区：
文献类型：
--
作者：
Duarte, LC;Carvalheiro, F;Gírio, FM
汉森德巴利酵母是一种高产多元醇酵母菌，可用于木质纤维水解物的改良。因此，建立其对水解物中发现的代谢抑制剂的耐受性是非常有意义的。我们研究了选定的脂肪酸、酚类化合物和糠醛的影响。在浓度为6.0g/L时，醋酸有利于生物量的产生，甲酸的毒性大于醋酸，并诱导木糖醇的积累(最高产量为0.21g/g木糖)。所有受试酚类物质都显著降低了比生长速率。对苯二酚、丁香醛和4-甲基儿茶酚的毒性增加，并与化合物的疏水性有关。增加呋喃甲醛的用量会导致滞后期延长，并对比生长速率和生物量生产力产生不利影响。
Debaryomyces hansenii is a polyol overproducing yeast that can have a potential use for upgrading lignocellulosic hydrolysates. Therefore, the establishment of its tolerance to metabolic inhibitors found in hydrolysates is of major interest. We studied the effects of selected aliphatic acids, phenolic compounds, and furfural. Acetic acid favored biomass production for concentrations < 6.0 g/L. Formic acid was more toxic than acetic acid and induced xylitol accumulation (maximum yield of 0.21 g/g of xylose). All tested phenolics strongly decreased the specific growth rate. Increased toxicity was found for hydroquinone, syringaldehyde, and 4-methylcatechol and was correlated to the compound's hydrophobicity. Increasing the amount of furfural led to longer lag phases and had a detrimental effect on specific growth rate and biomass productivity.