Experimental analysis of neuronal dynamics in cultured cortical networks and transitions between different patterns of activity
Experimental analysis of neuronal dynamics in cultured cortical networks and transitions between different patterns of activity
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DOI：
10.1007/s004220050376
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发表时间：
1997-08-01
影响因子：
1.9
通讯作者：
Kawana, A
中科院分区：
文献类型：
--
作者：
Canepari, M;Bove, M;Kawana, A
对生物网络动力学的实验研究是理解神经系统如何工作的基本一步。通过使用平面电极阵列的多位点记录技术，研究了皮层神经元培养网络中的自发活动。在这些网络中，研究了不同细胞外溶液存在下的时空放电模式。当细胞外 Ca2+ 浓度从 0.1 mM 增加到 1 mM 时，观察到从异步放电动力学到同步放电动力学的转变。细胞外Mg2+的添加降低了任何Ca2+浓度下的自发活动，并且细胞外K+浓度的增加增强了周期性同步爆发的频率。 N-甲基-D-天冬氨酸（NMDA）和非NMDA谷氨酸受体拮抗剂抑制同步活动，对数据进行了时空分析，并对网络的同步性和周期性等特性进行了量化，以阐明网络内在参数的变化如何引起神经动力学的结构转变。这项实验研究是研究神经网络计算特性的一种可能方法。
Experimental investigation of the dynamics of biological networks is a fundamental step towards understanding how the nervous system works. Spontaneous activity in cultured networks of cortical neurons has been investigated by using a multisite recording technique with planar electrode arrays. In these networks, the spatiotemporal firing patterns were studied in the presence of different extracellular solutions. Transitions from asynchronous firing dynamics to synchronous firing dynamics were observed when the extracellular Ca2+ concentration was increased from 0.1 mM to 1 mM. Addition of extracellular Mg2+ reduced the spontaneous activity at any Ca2+ concentration, and an increase in the extracellular K+ concentration enhanced the frequency of periodical synchronous bursts. N-methyl-D-aspartate (NMDA) and non-NMDA glutamate receptor antagonists inhibited synchronous activity, A spatiotemporal analysis of the data has been performed, and the properties of the network such as the synchronization and the periodicity have been quantified in order to clarify how variations of intrinsic parameters of the network can induce structural transitions in the neural dynamics. This experimental study is a possible approach to investigate the computational properties of a neuronal network.