Association between the effacing (eae) gene and the Shiga-like toxin-encoding genes in Escherichia coli isolates from cattle.
Association between the effacing (eae) gene and the Shiga-like toxin-encoding genes in Escherichia coli isolates from cattle.
复制标题
牛大肠杆菌中的擦除 (eae) 基因与志贺样毒素编码基因之间的关联。
DOI：
10.2460/ajvr.1993.54.07.1064
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发表时间：
1993
影响因子：
1
通讯作者：
P. Pohl
中科院分区：
文献类型：
--
作者：
J. Mainil;E. Jacquemin;A. Kaeckenbeeck;P. Pohl
用7种基因探针对296株从腹泻犊牛粪便或肠道中分离的大肠杆菌进行杂交。一个探针（eae探针）来自eae基因，该基因编码一种蛋白质，该蛋白质参与由称为附着和消除大肠杆菌（AEEC）的细菌群消除肠上皮细胞微绒毛，2个探针来自编码由verocytotoxic大肠杆菌（VTEC）产生的志贺样毒素（shiga-like toxins，SHG）1和2的基因。其他4个探针来自与肠粘附性大肠杆菌（EAEC）对培养细胞的粘附特性相关的DNA序列（EAF探针用于局部粘附模式，探针F1845和AIDA-1用于扩散粘附模式，以及Agg探针用于聚集粘附模式）。eae探针的杂交结果与先前发表的微绒毛消失的表型结果一致，除了8个分离株中的1个。后者以前被报道为隐去小牛肠上皮细胞的微绒毛，但eae探针阴性。两类分离物与eae探针杂交。第一类的成员（60个分离株）另外用1个或两个探针产生阳性信号（VTEC-AEEC分离株）。与eae和SLT 1探针杂交的分离株是最常见的：56株分离株（即所有VTEC-AEEC的93%）。第二类（10个分离株）的成员未能与任何一种荧光探针（非VTEC-AEEC分离株）杂交。（250字处删节）
Two hundred ninety-six Escherichia coli isolates from feces or intestines of calves with diarrhea were hybridized with 7 gene probes. One probe (the eae probe) was derived from the eae gene coding for a protein involved in the effacement of the enterocyte microvilli by the group of bacteria called attaching and effacing E coli (AEEC), and 2 probes were derived from genes coding for the Shiga-like toxins (SLT) 1 and 2 produced by the verocytotoxic E coli (VTEC). The other 4 probes were derived from DNA sequences associated with the adhesive properties of enteroadherent E coli (EAEC) to cultured cells (the EAF probe for the localized adherence pattern, probes F1845 and AIDA-1 for the diffuse adherence pattern, and the Agg probe for the aggregative adherence pattern). Hybridization results for the eae probe were in agreement, for all but 1 of the 8 isolates, with previously published phenotypic results of microvilli effacement. The latter was previously reported as effacing the microvilli of calf enterocytes, but was eae probe-negative. Two classes of isolates hybridized with the eae probe. Members of a first class (60 isolates) additionally produced a positive signal with 1 or both of the SLT probes (VTEC-AEEC isolates). Isolates hybridizing with the eae and the SLT1 probes were the most frequent: 56 isolates (ie, 93% of all VTEC-AEEC). Members of the second class (10 isolates) failed to hybridize with either SLT probe (non-VTEC-AEEC isolates).(ABSTRACT TRUNCATED AT 250 WORDS)