Helicobacter heilmannii sp nov., isolated from feline gastric mucosa
Helicobacter heilmannii sp nov., isolated from feline gastric mucosa
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DOI：
10.1099/ijs.0.029207-0
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发表时间：
2012-02-01
影响因子：
2.8
通讯作者：
Haesebrouck, F.
中科院分区：
文献类型：
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作者：
Smet, A.;Flahou, B.;Haesebrouck, F.
采用多相分类方法对从猫胃黏膜分离的3株革兰氏阴性嗜气微细菌ASB1(T)、ASB2和ASB3进行了研究。菌株在37℃微氧条件下双相培养板上生长，表现出脲酶、氧化酶和过氧化氢酶活性。它们也能在干燥的琼脂板上生长。基于16S rRNA基因序列分析，ASB1(T)、ASB2和ASB3被鉴定为幽门螺杆菌属，序列与此前在猫或犬胃粘膜检测到的6个相关幽门螺杆菌种felobacter、bizzozeronii幽门螺杆菌、‘Candidatus helmannii Helicobacter’、cynogastricus幽门螺杆菌、baculiformis幽门螺杆菌和salomonis幽门螺杆菌具有98% ~ 99%的相似性。对hsp60部分基因的测序表明，ASB1(T)、ASB2和ASB3在猫和犬幽门螺杆菌中构成一个独立的分类群。ASB1(T)、ASB2和ASB3的脲酶基因序列与“候选海门螺杆菌”的相似度约为91%。蛋白谱分析、缺乏碱性磷酸酶活性和其他一些生化特征也使菌株ASB1(T)、ASB2和ASB3与其他猫或犬胃源幽门螺杆菌种类区分开来。多相分类学研究结果表明，培养的分离株构成了一个新的分类群，与先前在人类、野生猫科动物、猫和狗的胃中发现的“Candidatus Helicobacter heilmannii”相对应。建议将这些分离株命名为海尔曼螺杆菌；型应变为ASB1(T) =DSM 23983(T)=LMG 26292(T)。
Three Gram-negative, microaerophilic bacteria, strains ASB1(T), ASB2 and ASB3, with a corkscrew-like morphology isolated from the gastric mucosa of cats were studied using a polyphasic taxonomic approach. The isolates grew on biphasic culture plates under microaerobic conditions at 37 degrees C and exhibited urease, oxidase and catalase activities. They were also able to grow in colonies on dry agar plates. Based on 16S rRNA gene sequence analysis, ASB1(T), ASB2 and ASB3 were identified as members of the genus Helicobacter and showed 98 to 99% sequence similarity to strains of Helicobacter felis, Helicobacter bizzozeronii, 'Candidatus Helicobacter heilmannii', Helicobacter cynogastricus, Helicobacter baculiformis and Helicobacter salomonis, six related Helicobacter species previously detected in feline or canine gastric mucosa. Sequencing of the partial hsp60 gene demonstrated that ASB1(T), ASB2 and ASB3 constitute a separate taxon among the feline and canine Helicobacter species. The urease gene sequences of ASB1(T), ASB2 and ASB3 showed approximately 91% similarity to those of 'Candidatus Helicobacter heilmannii'. Protein profiling, the absence of alkaline phosphatase activity and several other biochemical characteristics also allowed strains ASB1(T), ASB2 and ASB3 to be differentiated from other Helicobacter species of feline or canine gastric origin. The results of this polyphasic taxonomic study show that the cultured isolates constitute a new taxon corresponding to 'Candidatus Helicobacter heilmannii', which was previously demonstrated in the stomach of humans, wild felidae, cats and dogs. The name Helicobacter heilmannii sp. nov. is proposed for these isolates; the type strain is ASB1(T) (=DSM 23983(T)=LMG 26292(T)).