Development of the pancreas in medaka
Development of the pancreas in medaka
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DOI：
10.1016/s0925-4773(02)00190-9
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发表时间：
2002-09-01
影响因子：
2.6
通讯作者：
Scharfmann, R
中科院分区：
文献类型：
--
作者：
Assouline, B;Nguyen, V;Scharfmann, R
在目前的工作中，我们对青鳉（Oryzias latipes）的胰腺器官发生进行了研究，青鳉是一种硬骨鱼，作为发育生物学的实验系统具有多种优势。我们证明胰腺是由从肠上皮的背侧和腹侧出芽的三个原基发育而来。然后这些芽融合形成一个被外分泌组织包围的内分泌岛。有趣的是，内分泌组织仅由背芽形成。接下来，我们分析了与前脑文库中的 cDNA 杂交的青鳉集合。我们发现在胰腺区域表达的新克隆表明青鳉可用于定义在胰腺区域表达的新基因，这些基因遵循特定的空间和时间表达模式。 (C) 2002 Elsevier Science Ireland Ltd. 保留所有权利。
In the present work, pancreatic organogenesis has been studied in the medaka (Oryzias latipes), a teleost fish with several advantages as an experimental system in developmental biology. We demonstrated that the pancreas develops from three primordia budding from the dorsal and ventral faces of the gut epithelium. Such buds then fuse to form a single endocrine islet surrounded by exocrine tissue. Interestingly, the endocrine tissue forms only from the dorsal bud. We next analyzed a collection of medakas that had been hybridized with cDNAs derived from an anterior brain library. We found new clones expressed in the pancreatic region demonstrating that the medaka can be used to define new genes expressed in the pancreatic region that follow a specific spatial and temporal pattern of expression. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.