Mapping the reaction of peroxynitrite with CO2: energetics, reactive species, and biological implications.
Mapping the reaction of peroxynitrite with CO2: energetics, reactive species, and biological implications.
复制标题
绘制过氧亚硝酸盐与 CO2 的反应：能量学、活性物质和生物学意义。
DOI：
10.1021/tx020004c
复制
发表时间：
2002
影响因子：
4.1
通讯作者：
W. Pryor
中科院分区：
文献类型：
--
作者：
G. Squadrito;W. Pryor
一氧化氮是一种简单的自由基，在细胞调节和功能中起关键作用(1)。一氧化氮和超氧自由基之间的相互作用导致过氧亚硝酸盐的形成，特别是在一氧化氮和/或超氧化物过量产生的时期。贝克曼等人(3)最初提出的过氧亚硝酸盐的形成与病理有关。为了了解过氧亚硝酸盐的反应机理，研究工作最初主要集中在过氧亚硝酸盐与零级动力学底物（如二甲基亚砜和脱氧核糖）的反应，与二级速率常数相对较小的底物（如蛋氨酸和抗坏血酸）的反应，甚至一些更活泼的底物，如硫醇（3-6）。虽然这些实验提供了重要的机理信息，但在生理条件下，过氧亚硝酸盐与这些底物的反应不能与过氧亚硝酸盐与CO2的反应竞争。过氧亚硝酸盐和碳酸氢盐缓冲液之间的快速反应最早是由Keith和Powell报道的(7)，但这一观察的意义是
Nitric oxide is a simple free radical with pivotal roles in cell regulation and function (1). The interaction between nitric oxide and the superoxide radical results in the formation of peroxynitrite1 (2), particularly during episodes of excessive production of nitric oxide and/or superoxide. The formation of peroxynitrite, as originally proposed by Beckman et al.(3), has been linked to pathology. Research efforts directed to understand the mechanism of reaction of peroxynitrite were initially focused primarily on the reactions of peroxynitrite with substrates with zero order kinetics (eg, dimethyl sulfoxide and deoxyribose), with substrates with relatively small second-order rate constants (eg, methionine and ascorbate), and even a few more reactive substrates, such as thiols (3-6). Although these experiments afforded important mechanistic information, the reactions of peroxynitrite with these substrates cannot compete with the reaction of peroxynitrite with CO2 under physiological conditions. The fast reaction between peroxynitrite and bicarbonate buffers was first reported by Keith and Powell (7) but the implications of this observation were