Osmotic-Tension-Induced Membrane Lateral Organization
Osmotic-Tension-Induced Membrane Lateral Organization
复制标题
渗透张力诱导的膜横向组织
DOI：
10.1021/acs.langmuir.9b03893
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发表时间：
2020
期刊：
影响因子：
3.9
通讯作者：
Tsutomu Hamada
中科院分区：
文献类型：
--
作者：
Nichaporn Wongsirojkul;Naofumi Shimokawa;Pakorn Opaprakasit;Masahiro Takagi;Tsutomu Hamada
表现为时相分离的脂筏组织的改变对于细胞过程，如信号和运输，是重要的。脂膜的这种行为和动力学可以受到外界刺激的影响，包括物理刺激和化学刺激。在本研究中，我们重点研究了渗透张力诱导的相分离。用荧光显微镜定量研究了不同温度下渗透压对二油酰磷胆碱(DOPC)/二棕榈酰磷胆碱(DPPC)/胆固醇(CHOL)囊泡相行为的影响。我们测定了三元相图，发现张力导致了混相温度的移动。胆固醇在决定这种转变的程度上起着关键作用。此外，我们还发现渗透张力可以提高线的张力。讨论了渗透压诱导相分离的物理化学机理。
Alteration of lipid raft organization manifesting as phase separation is important for cellular processes, such as signaling and trafficking. Such behaviors and dynamics of lipid membranes can be affected by external stimuli including both physical and chemical stimuli. In this study, we focused on osmotic-tension-induced phase separation. The effects of osmotic tension on the phase behaviors of vesicles consisting of dioleoylphosphocholine (DOPC)/dipalmitoylphosphocholine (DPPC)/cholesterol (Chol) were quantitatively studied at different temperatures by fluorescence microscopy. We determined the ternary phase diagrams and found that tension leads to a shift in the miscibility temperature. Cholesterol plays a key role in determining the extent of this shift. In addition, we found that osmotic tension can enhance the line tension. The physicochemical mechanism of osmotic-pressure-induced phase separation is discussed.