NEOVASCULAR RESPONSES INDUCED BY CULTURED AORTIC ENDOTHELIAL-CELLS
NEOVASCULAR RESPONSES INDUCED BY CULTURED AORTIC ENDOTHELIAL-CELLS
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DOI：
10.1002/jcp.1041140308
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发表时间：
1983-01-01
影响因子：
5.6
通讯作者：
SCHWARTZ, SM
中科院分区：
文献类型：
--
作者：
HARRISHOOKER, SA;GAJDUSEK, CM;SCHWARTZ, SM
用微载体珠体分别接种牛主动脉内皮细胞、血管平滑肌细胞、Swiss和BALB/c[小鼠]3T3细胞及人二倍体成纤维细胞，观察鸡胚绒毛尿囊膜新生血管形成情况。对新生血管的定量分析表明，与无细胞的微珠相比，内皮细胞反应的血管数量增加了3.5倍，而平滑肌细胞的反应增加了2倍。皮肤成纤维细胞和瑞士3T3细胞没有引起类似的反应。内皮细胞诱导的显著血管生成反应的特征是与对照组相比，总血管长度增加了137%，平均血管面积增加了35%。血管生成因子所需的两个特性，刺激细胞迁移和增殖，也可以在细胞培养系统中使用内皮细胞条件培养液来证明。牛主动脉内皮细胞培养上清液可刺激血管内皮细胞DNA合成、增殖和迁移。此外，在绒毛尿囊膜实验中，来自内皮细胞和平滑肌细胞的条件培养液都产生了血管生成反应，这与生长在微载体珠上的完整细胞产生的血管生成反应相当。类似的反应在其他细胞类型的条件培养液中并不明显。这些结果表明内皮细胞和内皮细胞产物在调节血管生长方面具有潜在的重要性。[讨论了内皮细胞在伤口愈合中的作用。]。
Neovascularization was studied in the chorioallantoic membrane of the chick embryo after implantation of bovine aortic endothelial and smooth muscle cells, Swiss and BALB/c [mice] 3T3 cells and human diploid fibroblasts cultured separately on microcarrier beads. Quantitative analysis of neovascularization indicated a 3 1/2-fold increase in the number of blood vessels responding to endothelial cells while smooth muscle cells induced a 2-fold increase when compared to the response of beads without cells. Skin fibroblasts and Swiss 3T3 cells did not elicit a comparable response. The marked angiogenic response induced by endothelial cells was characterized by a 137% increase in total vessel length and a 35% increase in average vessel area when compared to controls. Two of the properties required for an angiogenesis factor, stimulation of cellular migration and proliferation, can also be demonstrated using endothelial cell-conditioned medium in cell culture systems. Medium from cultured bovine aortic endothelium stimulates DNA synthesis, proliferation and migration of smooth muscle cells. In addition, conditioned media from both endothelial cells and smooth muscle cells produced an angiogenic response in the chorioallantoic membrane assay, which was comparable to that produced by intact cells growing on microcarrier beads. Similar responses were not evident with medium conditioned by other cell types. These results indicate the potential importance of endothelial cells and endothelial cell products in regulating blood vessel growth. [The involvement of endothelial cells in wound healing is discussed.].