Estrogen upregulates renal angiotensin II AT2 receptors
Estrogen upregulates renal angiotensin II AT2 receptors
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DOI：
10.1152/ajprenal.00145.2002
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发表时间：
2002-11-01
影响因子：
4.2
通讯作者：
Saavedra, JM
中科院分区：
文献类型：
--
作者：
Armando, I;Jezova, M;Saavedra, JM
AT（2）受体可能与AT（1）受体作用相反并与之平衡，它们的刺激具有有益的作用。我们发现雌性小鼠肾脏AT（2）受体的表达高于雄性小鼠。我们提出这样的表达是否可能是雌激素依赖性的问题。用免疫细胞化学和放射自显影法研究了雄性、雌性、去卵巢和雌激素处理的去卵巢小鼠肾脏AT（1）和AT（2）受体，用RT-PCR法研究了AT（2）受体mRNA，用RIA法研究了cAMP、cGMP和PGE（2）。AT（1）受体占优势。AT（2）受体分布于肾小球、髓射线、内髓和雌性肾被膜。AT（1）和AT（2）受体共定位于肾小球。雌性小鼠表达较少的肾小球AT（1）受体。卵巢切除术后，髓射线中的AT（1）受体和囊膜AT（2）受体减少。雌激素可使髓质射线中的AT（1）受体正常化，增加AT（2）受体，主要分布在被膜和内髓质，以及肾小球、髓质射线和外髓质内条。雌激素处理的去卵巢小鼠髓质中AT（2）受体mRNA表达增加，cGMP含量降低，PGE（2）含量增加。我们认为雌激素的保护作用可能部分是通过增强AT（2）受体的刺激来介导的。
AT(2) receptors may act in opposition to and in balance with AT(1) receptors, their stimulation having beneficial effects. We found renal AT(2) receptor expression in female mice higher than in male mice. We asked the question of whether such expression might be estrogen dependent. In male, female, ovariectomized, and estrogen-treated ovariectomized mice, we studied renal AT(1) and AT(2) receptors by immunocytochemistry and autoradiography, AT(2) receptor mRNA by RT-PCR, and cAMP, cGMP, and PGE(2) by RIA. AT(1) receptors predominated. AT(2) receptors were present in glomeruli, medullary rays, and inner medulla, and in female kidney capsule. AT(1) and AT(2) receptors colocalized in glomeruli. Female mice expressed fewer glomerular AT(1) receptors. Ovariectomy decreased AT(1) receptors in medullary rays and capsular AT(2) receptors. Estrogen administration normalized AT(1) receptors in medullary rays and increased AT(2) receptors predominantly in capsule and inner medulla, and also in glomeruli, medullary rays, and inner stripe of outer medulla. In medullas of estrogen-treated ovariectomized mice there was higher AT(2) receptor mRNA, decreased cGMP, and increased PGE(2) content. We propose that the protective effects of estrogen may be partially mediated through enhancement of AT(2) receptor stimulation.