A comparison of soil microbial community structure, protozoa and nematodes in field plots of conventional and genetically modified maize expressing the Bacillus thuringiens is CryIAb toxin
A comparison of soil microbial community structure, protozoa and nematodes in field plots of conventional and genetically modified maize expressing the Bacillus thuringiens is CryIAb toxin
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DOI：
10.1007/s11104-005-1093-2
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发表时间：
2005-08-01
期刊：
影响因子：
4.9
通讯作者：
Krogh, PH
中科院分区：
文献类型：
--
作者：
Griffiths, BS;Caul, S;Krogh, PH
在欧洲三个地点（丹麦、法国东部、法国西南部）建立了转基因玉米（Zea mays L.）表达CryIAb苏云金芽孢杆菌毒素（Bt）、近等基因非Bt栽培品种、另一种常规玉米栽培品种和草。在两年（2002年、2003年）的播种（5月）和收获（10月）时对丹麦的土壤进行取样;在2002年收获时对法国东部的土壤进行取样，在2003年播种和收获时对法国西南部的土壤进行取样。通过群落级生理概况分析（CLPP）和磷脂脂肪酸分析（PLFA）分析了样本的微生物群落结构（仅2003年样本），并在所有样本中分析了原生动物和线虫。一个网站内的个体差异导致：更大的线虫数量在三个场合下比玉米草;不同的线虫种群下的常规玉米品种一次;和两次时，有一个减少原生动物种群下Bt玉米相比，非Bt玉米。微生物群落结构内的网站只变化与草相比，玉米，与一个发生的CLPP玉米品种之间的变化（Bt与传统品种）。对所有三个地点的Bt玉米与非Bt玉米的总体比较仅显示线虫的差异，Bt玉米下的线虫种群较小。线虫群落结构不同，在每个网站和Bt的效果并不局限于特定的线虫类群。Bt玉米的影响很小，在这些农业系统中预期的正常变化范围内。
Field trials were established at three European sites (Denmark, Eastern France, South-West France) of genetically modified maize (Zea mays L.) expressing the CryIAb Bacillus thuringiensis toxin (Bt), the near-isogenic non-Bt cultivar, another conventional maize cultivar and grass. Soil from Denmark was sampled at sowing (May) and harvest (October) over two years (2002, 2003); from E France at harvest 2002, sowing and harvest 2003; and from SW France at sowing and harvest 2003. Samples were analysed for microbial community structure (2003 samples only) by community-level physiological-profiling (CLPP) and phospholipid fatty acid analysis (PLFA), and protozoa and nematodes in all samples. Individual differences within a site resulted from: greater nematode numbers under grass than maize on three occasions; different nematode populations under the conventional maize cultivars once; and two occasions when there was a reduced protozoan population under Bt maize compared to non-Bt maize. Microbial community structure within the sites only varied with grass compared to maize, with one occurrence of CLPP varying between maize cultivars (Bt versus a conventional cultivar). An overall comparison of Bt versus non-Bt maize across all three sites only revealed differences for nematodes, with a smaller population under the Bt maize. Nematode community structure was different at each site and the Bt effect was not confined to specific nematode taxa. The effect of the Bt maize was small and within the normal variation expected in these agricultural systems.