Deterministic Lateral Displacement: The Next-Generation CAR T-Cell Processing?
Deterministic Lateral Displacement: The Next-Generation CAR T-Cell Processing?
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DOI：
10.1177/2472630317751214
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发表时间：
2018-08-01
期刊：
影响因子：
2.7
通讯作者：
Ward, Tony
中科院分区：
文献类型：
--
作者：
Campos-Gonzalez, Roberto;Skelley, Alison M.;Ward, Tony
Reliable cell recovery and expansion are fundamental to the successful scale-up of chimeric antigen receptor (CAR) T cells or any therapeutic cell-manufacturing process. Here, we extend our previous work in whole blood by manufacturing a highly parallel deterministic lateral displacement (DLD) device incorporating diamond microposts and moving into processing, for the first time, apheresis blood products. This study demonstrates key metrics of cell recovery (80%) and platelet depletion (87%), and it shows that DLD T-cell preparations have high conversion to the T-central memory phenotype and expand well in culture, resulting in twofold greater central memory cells compared to Ficoll-Hypaque (Ficoll) and direct magnetic approaches. In addition, all samples processed by DLD converted to a majority T-central memory phenotype and did so with less variation, in stark contrast to Ficoll and direct magnetic prepared samples, which had partial conversion among all donors (
