A survey of ovary-, testis-, and soma-biased gene expression in Drosophila melanogaster adults.
A survey of ovary-, testis-, and soma-biased gene expression in Drosophila melanogaster adults.
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DOI：
10.1186/gb-2004-5-6-r40
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发表时间：
2004
期刊：
影响因子：
12.3
通讯作者：
Oliver B
中科院分区：
文献类型：
--
作者：
Parisi M;Nuttall R;Edwards P;Minor J;Naiman D;Lü J;Doctolero M;Vainer M;Chan C;Malley J;Eastman S;Oliver B
果蝇性别偏向转录的全球分析表明，性别之间存在广泛的差异表达。大多数性别差异表达是由于生殖细胞和几乎所有的基因与生殖细胞表达显示性别偏见。两性异形导致形成两种类型的具有专门生殖作用的个体，并且在生殖细胞和性腺中最为明显。我们使用31，464个元件的FlyGEM微阵列对性别之间的转录进行了全球分析，以确定基因组的哪些部分显示性别偏向表达，哪些组织表达这些基因，这些基因的预测功能，以及这些基因在基因组中的位置。对雌性和雄性（有和无性腺）、解剖的睾丸和卵巢、具有基因消融生殖系的雌性和雄性以及性转化果蝇进行采样。使用任何一个标准，我们发现广泛的性别偏见的表达在成年人。大多数性别差异基因表达的病例可归因于生殖细胞。还有一大类基因具有体细胞偏向性表达。几乎没有生殖系偏好表达，表明几乎所有具有生殖系表达的基因也显示出性别偏好。Monte Carlo模拟表明，一些具有性别偏好表达的基因在基因组中是非随机分布的。
A global analysis of sex-biased transcription in Drosophila shows extensive differential expression between the sexes. Most sex-differential expression is due to germ cells and nearly all genes with germline expression show sex-bias. Sexual dimorphism results in the formation of two types of individuals with specialized reproductive roles and is most evident in the germ cells and gonads. We have undertaken a global analysis of transcription between the sexes using a 31,464 element FlyGEM microarray to determine what fraction of the genome shows sex-biased expression, what tissues express these genes, the predicted functions of these genes, and where these genes map onto the genome. Females and males (both with and without gonads), dissected testis and ovary, females and males with genetically ablated germlines, and sex-transformed flies were sampled. Using any of a number of criteria, we find extensive sex-biased expression in adults. The majority of cases of sex differential gene expression are attributable to the germ cells. There is also a large class of genes with soma-biased expression. There is little germline-biased expression indicating that nearly all genes with germline expression also show sex-bias. Monte Carlo simulations show that some genes with sex-biased expression are non-randomly distributed in the genome.