Temporal and spatial expression patterns of TUB9, a β-tubulin gene of Arabidopsis thaliana
Temporal and spatial expression patterns of TUB9, a β-tubulin gene of Arabidopsis thaliana
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DOI：
10.1023/a:1011628024798
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发表时间：
2001-10-01
影响因子：
5.1
通讯作者：
Carter, JV
中科院分区：
文献类型：
--
作者：
Cheng, ZG;Snustad, DP;Carter, JV
将拟南芥9-微管蛋白基因（TUB 9）的5 '侧翼区片段与大肠杆菌β-葡萄糖醛酸酶（GUS）基因的编码区融合，构建了嵌合基因转基因植株，研究了TUB 9表达的时空模式。含有至少800 bp TUB 9 5 '侧翼DNA的嵌合基因主要在花组织中表达，在花粉、伸长的花粉管和胚珠中观察到高水平的表达。报告基因在胚珠中的表达在受精时停止。原位杂交用于验证报告基因在转基因植物花粉中的表达是内源TUB 9基因表达模式的代表。原位杂交也提供了新的见解TUB 9在胚珠中的转录积累。TUB 9的可能作用和微管蛋白基因的大部分非重叠表达模式的功能意义进行了讨论。
Transgenic plants carrying chimeric genes composed of segments of the 5'-flanking region of the Arabidopsis 9-tubulin gene (TUB9) fused to the coding region of the beta -glucuronidase (GUS) gene of Escherichia coli were used to investigate the temporal and spatial patterns of TUB9 expression. Chimeric genes that contained at least 800 bp of TUB9 5'-flanking DNA were expressed primarily in floral tissues, with high levels of expression observed in pollen, elongating pollen tubes and ovules. The expression of the reporter genes in ovules ceased at the time of fertilization. In situ hybridization was used to verify that the reporter gene expression in pollen of transgenic plants is representative of the patterns of expression of the endogenous TUB9 gene. In situ hybridization also provided new insight into TUB9 transcript accumulation in ovules. The possible role of TUB9 and the functional implication of the largely non-overlapping expression patterns of tubulin genes are discussed.