Silencing OsHI-LOX makes rice more susceptible to chewing herbivores, but enhances resistance to a phloem feeder
Silencing OsHI-LOX makes rice more susceptible to chewing herbivores, but enhances resistance to a phloem feeder
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DOI：
10.1111/j.1365-313x.2009.03988.x
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发表时间：
2009-11-01
期刊：
影响因子：
7.2
通讯作者：
Lou, Yonggen
中科院分区：
文献类型：
--
作者：
Zhou, Guoxin;Qi, Jinfeng;Lou, Yonggen
茉莉酸（JA）途径在植物抗虫防御反应中起着重要作用.一些取食韧皮部的昆虫也诱导水杨酸（SA）途径，从而抑制植物的JA反应。这些现象在双子叶植物中已经得到了很好的研究，但在单子叶植物中却知之甚少。我们克隆了水稻叶绿体定位的2型13-脂氧合酶基因OsHI-LOX，其转录物响应于水稻二化螟（Chilo suppressalis）和水稻褐飞虱（BPH）的取食以及机械创伤和JA处理而上调。反义表达OsHI-LOX（as-lox）可降低SSB和BPH诱导的JA和胰蛋白酶抑制剂（TrypPI）水平，提高稻纵卷叶螟（Cnaphalocrocis medinalis）和水稻黑胫病（SBB）幼虫的生长性能，并增加SSB和LF幼虫对稻纵卷叶螟的危害。与此相反，BPH，韧皮部喂养的草食动物，表现出偏好的解决和产卵的野生型植物，他们消耗更多，生存得更好，比作为-lox植物。增强抗氧化植物BPH侵染与较高水平的BPH诱导的H2 O2和SA，以及增加过敏反应样细胞死亡。这些结果表明OsHI-LOX参与了植食性昆虫诱导的JA生物合成，并在控制水稻对咀嚼型和韧皮部取食型植食性昆虫的抗性方面发挥了不同的作用。JA活性的抑制导致对昆虫的抗性增加的观察表明，在单子叶植物中的广义植物防御模型的修订是必要的，并可能有助于开发新的策略，以保护水稻免受虫害。
P>The jasmonic acid (JA) pathway plays a central role in plant defense responses against insects. Some phloem-feeding insects also induce the salicylic acid (SA) pathway, thereby suppressing the plant's JA response. These phenomena have been well studied in dicotyledonous plants, but little is known about them in monocotyledons. We cloned a chloroplast-localized type 2 13-lipoxygenase gene of rice, OsHI-LOX, whose transcripts were up-regulated in response to feeding by the rice striped stem borer (SSB) Chilo suppressalis and the rice brown planthopper (BPH) Niaparvata lugens, as well as by mechanical wounding and treatment with JA. Antisense expression of OsHI-LOX (as-lox) reduced SSB- or BPH-induced JA and trypsin protease inhibitor (TrypPI) levels, improved the larval performance of SBB as well as that of the rice leaf folder (LF) Cnaphalocrocis medinalis, and increased the damage caused by SSB and LF larvae. In contrast, BPH, a phloem-feeding herbivore, showed a preference for settling and ovipositing on WT plants, on which they consumed more and survived better than on as-lox plants. The enhanced resistance of as-lox plants to BPH infestation correlated with higher levels of BPH-induced H2O2 and SA, as well as with increased hypersensitive response-like cell death. These results imply that OsHI-LOX is involved in herbivore-induced JA biosynthesis, and plays contrasting roles in controlling rice resistance to chewing and phloem-feeding herbivores. The observation that suppression of JA activity results in increased resistance to an insect indicates that revision of the generalized plant defense models in monocotyledons is required, and may help develop novel strategies to protect rice against insect pests.