Environmental stimuli drive a transition from cooperation to competition in synthetic phototrophic communities
Environmental stimuli drive a transition from cooperation to competition in synthetic phototrophic communities
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DOI：
10.1038/s41564-019-0567-6
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发表时间：
2019-08
影响因子：
28.3
通讯作者：
C. Zuñiga;Chien-Ting Li;Geng Yu;Mahmoud M. Al-Bassam;Tingting Li;Liqun Jiang;L. Zaramela;M. Guarnieri;M. Betenbaugh;K. Zengler
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文献类型：
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作者：
C. Zuñiga;Chien-Ting Li;Geng Yu;Mahmoud M. Al-Bassam;Tingting Li;Liqun Jiang;L. Zaramela;M. Guarnieri;M. Betenbaugh;K. Zengler
光合藻类或蓝细菌和异养细菌或真菌的光养群落在整个环境中普遍存在。成员之间的相互作用如何促进光养群落的恢复力和适应度尚不完全清楚。在这里，我们将宏转录组学、代谢组学和表型分析与计算模型相结合，以揭示合成群落中代谢物的条件依赖性分泌和交叉喂养。我们发现成员之间的相互作用是高度动态的，并且是由有机和无机营养素的可用性驱动的。环境因素（例如氨浓度）通过将成员从合作转变为竞争来影响群落稳定性。此外，整体适应性取决于基因型，精简的基因组改善了整个群落的生长。我们的机制框架提供了对这些普遍存在的微生物关联的环境和遗传扰动的生理学和代谢反应的见解。
Phototrophic communities of photosynthetic algae or cyanobacteria and heterotrophic bacteria or fungi are pervasive throughout the environment. How interactions between members contribute to the resilience and affect the fitness of phototrophic communities is not fully understood,. Here, we integrated metatranscriptomics, metabolomics and phenotyping with computational modelling to reveal condition-dependent secretion and cross-feeding of metabolites in a synthetic community. We discovered that interactions between members are highly dynamic and are driven by the availability of organic and inorganic nutrients. Environmental factors, such as ammonia concentration, influenced community stability by shifting members from collaborating to competing. Furthermore, overall fitness was dependent on genotype and streamlined genomes improved growth of the entire community. Our mechanistic framework provides insights into the physiology and metabolic response to environmental and genetic perturbation of these ubiquitous microbial associations.