Ribonuclease attenuates acute intestinal injury induced by intestinal ischemia reperfusion in mice
Ribonuclease attenuates acute intestinal injury induced by intestinal ischemia reperfusion in mice
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核糖核酸酶减轻小鼠肠道缺血再灌注引起的急性肠道损伤
DOI：
10.1016/j.intimp.2020.106430
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发表时间：
2020
影响因子：
5.6
通讯作者：
Liu Ke-Xuan
中科院分区：
文献类型：
--
作者：
Zhang Xi-Yang;Liang Hai-Su;Hu Jing-Juan;Wan Yan-Tong;Zhao Jin;Liang Guang-Tao;Luo Yu-Han;Liang Hao-Xuan;Guo Xiao-Qing;Li Cai;Liu Wei-Feng;Liu Ke-Xuan
核糖核酸酶（Ribonuclease，RNase）在包括缺血再灌注（ischemic reperfusion，I/R）在内的多种病理状态下具有器官保护作用，但其是否对肠I/R损伤具有保护作用及其机制尚不清楚。本研究旨在探讨核糖核酸酶对肠I/R损伤的影响及其机制。将32只野生型C57 BL/6 J成年雄性小鼠平均分为假手术组、假手术+ RNase组、I/R组和I/R + RNase组。肠I/R模型采用夹闭上级肠系膜动脉1 h，再灌注2 h。所有小鼠在不同时间点分别给予3个剂量的RNase或相同剂量的生理盐水。结果发现，肠I/R引起显著的肠损伤和细胞外RNA（exRNA）水平的增加。RNase可显著降低炎症细胞因子的产生，抑制肠细胞凋亡，下调肠组织Toll样受体3的表达。总之，exRNA增加可能导致成年小鼠肠I/R损伤，围手术期窗期RNase治疗可有效减轻肠I/R损伤。
Ribonuclease (RNase) reportedly exerts organ-protective effects in several pathological conditions, including ischemia reperfusion (I/R), but whether it can exhibit protective effect on intestinal I/R injury and potential mechanisms remain unknown. The present study was aimed to evaluate the effects of RNase on intestinal I/R injury and explore the underlying mechanisms. Thirty-two wild-type C57BL/6J adult male mice were evenly divided into a sham group, a sham + RNase group, an I/R group and an I/R + RNase group. Intestinal I/R was produced by clamping the superior mesenteric artery for 1 h followed by reperfusion for 2 h. All mice were treated with 3 doses of RNase or the same dosage of normal saline at different points. It was found that intestinal I/R caused significant intestinal injury and an increase in levels of extracellular RNAs (exRNAs). Treatment with RNase significantly reduced the inflammatory cytokine production, inhibited intestinal apoptosis and down-regulated the expression of toll like receptor 3 in intestinal tissues. In conclusion, increased exRNAs may contribute to intestinal I/R injury in adult mice, and RNase treatment during perioperative window is effective for attenuating intestinal I/R injury.