Molecular cloning and expression analysis of glutathione reductase gene in Chlamydomonas sp ICE-L from Antarctica
Molecular cloning and expression analysis of glutathione reductase gene in Chlamydomonas sp ICE-L from Antarctica
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DOI：
10.1016/j.margen.2011.11.001
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发表时间：
2012-03-01
期刊：
影响因子：
1.9
通讯作者：
Miao, Jin-Lai
中科院分区：
文献类型：
--
作者：
Ding, Yu;Liu, Ying;Miao, Jin-Lai
cDNA（GenBank ID：利用RT-PCR和RACE技术成功克隆了南极微藻Chlamydiumsp. ICE-L胞浆谷胱甘肽还原酶基因（ICE-LGR）。利用实时荧光定量PCR技术分析了不同盐度胁迫下ICE-LGR的表达模式。ICE-LGR cDNA全长1913 bp，开放阅读框（ORF）为1458 bp，编码485个氨基酸。推导的氨基酸序列与莱茵衣藻谷胱甘肽还原酶（GR）的同源性为79%。活性测定和mRNA表达分析结果表明，高盐和低盐条件下ICE-L细胞GR活性和表达水平均上调。这些结果表明，ICE-LGR可能在南极冰藻Chlamydiumsp. ICE-L适应极地高盐和低盐环境中发挥重要作用。这些结果为进一步研究ICE-LGR的分子机制提供了有价值的信息。(C)2011 Elsevier B. V.保留所有权利。
A cDNA (GenBank ID: GU395492) encoding cytosolic glutathione reductase (named ICE-LGR) in Antarctic microalgae Chlamydomonas sp. ICE-L was successfully cloned by RT-PCR and rapid amplification of cDNA ends technique (RACE). The expression patterns of ICE-LGR under different salinity stresses were determined by real-time PCR. ICE-LGR cDNA has 1913 bp nucleotides with an open reading frame (ORF) of 1458 bp, encoding 485 amino acid residues. The deduced amino acid sequence shows 79% homology with glutathione reductase (GR) of Chlamydomonas reinhardtii. Activity assessment and mRNA expression analysis results showed that activity and expression level of GR in ICE-L cells were up-regulated under either high or low salinity. Together, our results revealed that ICE-LGR might play an important role in Antarctic ice algae Chlamydomonas sp. ICE-L acclimatizing to polar high salinity environment as well as low salinity. These results provide us valuable information on further investigating the molecular mechanism of ICE-LGR. (C) 2011 Elsevier B.V. All rights reserved.