The effect of electrical deformation forces on the electropermeabilization of erythrocyte membranes in low- and high-conductivity media
The effect of electrical deformation forces on the electropermeabilization of erythrocyte membranes in low- and high-conductivity media
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DOI：
10.1007/s002329900387
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发表时间：
1998-06-01
影响因子：
2.4
通讯作者：
Zimmermann, U
中科院分区：
文献类型：
--
作者：
Sukhorukov, VL;Mussauer, H;Zimmermann, U
红细胞的电击穿诱导血红蛋白释放，其随着脉冲介质的电导率的降低而显著增加。这种效应可能是由麦克斯韦应力引起的电池上的瞬时、电导率相关的变形力（伸长或压缩）引起的。变形力施加在细胞的质膜上，其可以被视为由施加的DC电场脉冲诱导的瞬态偶极子。诱导偶极子产生于通过麦克斯韦-瓦格纳极化机制在细胞界面处积累的自由电荷。红细胞的极化响应的直流场脉冲估计从实验数据获得，通过使用两个互补的频域技术。由于细胞质具有高导电性，因此反应非常迅速。在宽的电导率范围内的电旋转和电变形光谱的测量产生的变形力的计算所需的信息和数据作为频率和外部电导率的函数，并用于计算的瞬态发展的变形力的应用过程中的DC场脉冲。这些计算表明，（i）电力之前，并伴随着膜充电（击穿电压）和（ii）在低电导率条件下，电拉伸力显着扩大的“电泄漏”在质膜产生的电击穿。
Electrical breakdown of erythrocytes induces hemoglobin release which increases markedly with decreasing conductivity of the pulse medium. This effect presumably results from the transient, conductivity-dependent deformation forces (elongation or compression) on the cell caused by Maxwell stress. The deformation force is exerted on the plasma membrane of the cell, which can be viewed as a transient dipole induced by an applied DC electric field pulse. The induced dipole arises from the free charges that accumulate at the cell interfaces via the Maxwell-Wagner polarization mechanism. The polarization response of erythrocytes to a DC field pulse was estimated from the experimental data obtained by using two complementary frequency-domain techniques. The response is very rapid, due to the highly conductive cytosol. Measurements of the electrorotation and electrodeformation spectra over a wide conductivity range yielded the information and data required for the calculation of the deformation force as a function of frequency and external conductivity and for the calculation of the transient development of the deformation forces during the application of a DC-field pulse. These calculations showed that (i) electric force precedes and accompanies membrane charging (up to the breakdown voltage) and (ii) that under low-conductivity conditions, the electric stretching force contributes significantly to the enlargement of "electroleaks" in the plasma membrane generated by electric breakdown.