Meiotic contraction of CAG repeats in Saccharomyces cerevisiae
Meiotic contraction of CAG repeats in Saccharomyces cerevisiae
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DOI：
10.1266/ggs.74.159
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发表时间：
1999-08-01
影响因子：
1.1
通讯作者：
Inoue, T
中科院分区：
文献类型：
--
作者：
Arai, N;Akiyama, R;Inoue, T
几种人类神经退行性疾病是由在减数分裂或配子发生期间发生的CAG重复序列的扩增引起的。我们预期克隆在酿酒酵母质粒中的CAG重复序列在减数分裂和孢子形成过程中可能会发生重复序列数量的变化。为了测试这种可能性，我们设计了一种新的方法来在体外改变CAG重复序列的数量，并从携带（CAG）（18）的质粒构建了携带（CAG）（39）、（CAG）（65）或（CAG）（123）的质粒。发现长GAG的收缩;重复频繁发生，而观察到少数扩张病例。当宿主细胞通过减数分裂生长时，两个方向的收缩同样增强。因此，我们的研究结果表明，长CAG重复序列是不稳定的减数分裂或配子发生在S。啤酒。
Several human neurodegenerative disorders are caused by expansion of CAG repeats that occurs during meiosis or gametogenesis. We anticipated that the CAG repeats cloned in a plasmid of Saccharomyces cerevisiae might undergo a change in the number of repeats during meiosis and sporulation. To test this possibility, we devised a new method to change in vitro the number of CAG repeats and constructed plasmids carrying (CAG)(39), (CAG)(65) or (CAG)(123) from a plasmid carrying (CAG)(18) We monitored the number of colonies showing an altered length of the repeat tracts during mitosis and meiotic growth. Contraction of long GAG; repeat was found to occur frequently, whereas a few cases of expansion were observed. The contraction was equally enhanced in both orientations when the host cells grew through meiosis. Thus, our results suggest that long CAG repeats are destabilized during meiosis or gametogenesis in S. cerevisiae.