Isolation and characterization of mammalian homologs of the Drosophila gene glial cells missing
Isolation and characterization of mammalian homologs of the Drosophila gene glial cells missing
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DOI：
10.1073/pnas.95.21.12364
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发表时间：
1998-10-13
影响因子：
11.1
通讯作者：
Anderson, DJ
中科院分区：
文献类型：
--
作者：
Kim, JS;Jones, BW;Anderson, DJ
果蝇的神经胶质细胞缺失（gcm）基因编码一种转录因子，该转录因子决定了神经胶质细胞和神经元命运之间的选择。我们在此报道了两个哺乳动物宝石同源物Gcm1和Gcm2的分离，并表征了它们在胚胎发育过程中的表达模式。尽管Gcm2在神经组织中表达水平较低，但这两个哺乳动物基因的主要表达位点都是非神经的，这表明哺乳动物同源基因的功能已经分化和多样化。然而，当Gcm基因异位表达时，Gcm1可以通过将假定的神经元转化为胶质细胞而在功能上替代果蝇的gem，因此，某些生化特性，尽管不是基因表达组织的特异性，已经通过Gcm基因家族的进化而被保存下来。
The glial cells missing (gcm) gene in Drosophila encodes a transcription factor that determines the choice between glial and neuronal fates. We report here the isolation of two mammalian gem homologs, Gcm1 and Gcm2, and the characterization of their expression patterns during embryonic development. Although Gcm2 Is expressed in neural tissues at a low level, the major sites of expression for both of the mammalian genes are nonneural, suggesting that the functions of the mammalian homologs have diverged and diversified. However, when expressed ectopically, Gcm1 can substitute functionally for Drosophila gem by transforming presumptive neurons into glia, Thus, certain biochemical properties, although not the specificity of the tissue in which the gene is expressed, have been conserved through the evolution of the Gcm gene family.