Two distinct actin networks drive the protrusion of migrating cells
Two distinct actin networks drive the protrusion of migrating cells
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DOI：
10.1126/science.1100533
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发表时间：
2004-09-17
期刊：
影响因子：
56.9
通讯作者：
Danuser, G
中科院分区：
文献类型：
--
作者：
Ponti, A;Machacek, M;Danuser, G
细胞迁移起始于肌动蛋白细胞骨架在前缘的延伸。荧光斑点显微镜电影迁移上皮细胞的计算分析表明，这一过程是由两个空间共定位，但运动学，动力学，分子和功能不同的肌动蛋白网络介导的。在前缘处组装的片足状网络，但在1至3微米内完全分解。它与细胞骨架的其余部分弱耦合，并促进前缘的随机伸出和缩回。生产性细胞的进步是第二个共定位网络，板层，其中肌动球蛋白收缩与基板粘附集成的功能。
Cell migration initiates by extension of the actin cytoskeleton at the leading edge. Computational analysis of fluorescent speckle microscopy movies of migrating epithelial cells revealed this process is mediated by two spatially colocalized but kinematically, kinetically, molecularly, and functionally distinct actin networks. A lamelhpodium network assembled at the leading edge but completely disassembled within 1 to 3 micrometers. It was weakly coupled to the rest of the cytoskeleton and promoted the random protrusion and retraction of the leading edge. Productive cell advance was a function of the second colocalized network, the lamella, where actomyosin contraction was integrated with substrate adhesion.