Integrated genetic linkage map based on UGMS and gSSR markers in Brassica rapa
Integrated genetic linkage map based on UGMS and gSSR markers in Brassica rapa
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DOI：
10.1016/j.scienta.2014.09.046
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发表时间：
2014-11
影响因子：
4.3
通讯作者：
Zhe Wang;Y. Ge;Jing Jing-Jing;Xinli Han;Z. Piao
中科院分区：
文献类型：
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作者：
Zhe Wang;Y. Ge;Jing Jing-Jing;Xinli Han;Z. Piao
简单序列重复序列（SSRs），如gSSRs和表达序列标签(EST)-SSRs，特别是用于unigene衍生微卫星（UGMS）的SSRs，由于其高度多态性、共显性和高效的植物基因组分析，被广泛用于构建芸苔种遗传图谱。本研究将309个gSSRs、226个UGMS和5个附加标记分别定位于油菜不同亚种的3个定位群体中的一个或多个，并进行比对，以构建完整的遗传图谱。在10个连锁组中共定位了540个标记，包括548个位点。综合连锁图谱的总长度为1086.6 cM，相邻标记间平均距离为1.98 cM。此外，通过将图谱中标记的引物序列与b的基因组序列比对，分析了标记的拷贝数。拉伯。为今后在重要经济性状的标记辅助选择中应用，确定了单拷贝标记。
Simple sequence repeats (SSRs) such as gSSRs and expressed sequence tag (EST)-SSRs, especially for UniGene-derived microsatellite (UGMS), are widely used in the construction of genetic maps ofBrassicaspecies, because they are highly polymorphic, co-dominant, and efficient for plant genome analysis. In this study, 309 gSSRs, 226 UGMS, and five additional markers were mapped in one or more of the three individual mapping populations derived from different subspecies ofBrassica rapaand aligned in order to construct a integrated genetic map. A total of 540 markers, including 548 loci, were mapped in 10 linkage groups. The total length of the integrated linkage map was 1086.6 cM with an average distance of 1.98 cM between adjacent markers. In addition, the copy numbers of the markers were analyzed by aligning the primer sequences of the markers in the map against the genome sequence ofB. rapa. Single-copy markers were identified for future applications in marker-assisted selection of important economic traits.