Targeted Nuclear Irradiation with an X-Ray Microbeam Enhances Total JC-1 Fluorescence from Mitochondria
Targeted Nuclear Irradiation with an X-Ray Microbeam Enhances Total JC-1 Fluorescence from Mitochondria
复制标题
x射线微束靶向核照射增强线粒体总JC-1荧光
DOI：
10.1667/rr15110.1
复制
发表时间：
2020-11-01
影响因子：
3.4
通讯作者：
Yokoya, Akinari
中科院分区：
文献类型：
--
作者：
Kaminaga, Kiichi;Hamada, Ryo;Yokoya, Akinari
几项研究表明，线粒体在辐射效应的多效性表现中起着至关重要的作用。虽然传统的全细胞照射损害了线粒体的功能，但亚细胞靶向辐射的影响尚未完全了解。在这个螺柱。用同步加速器X射线微束照射具有细胞周期指标的正常人二倍体细胞，并在照射后72小时内用JC-1定量线粒体膜电位。观察到细胞质照射使线粒体暂时增大！高膜电位区，而总线粒体面积没有显着变化。出乎意料的是，细胞核照射不仅促进了线粒体的类似增加！高膜电位区，但也在那些低膜电位，这引起了总线粒体面积的明显增加。增强的线粒体面积与低膜电位主要观察到G，细胞，表明核辐射在G，阶段调节细胞质的线粒体动力学，大概是通过DNA损伤的细胞核。(C)2020年：辐射研究学会
Several studies have demonstrated that mitochondria are critically involved in the pleiotropic manifestation of radiation effects. While conventional whole-cell irradiation compromises the function of mitochondria, the effects of subcellular targeted radiation are not yet fully understood. In this studs. normal human diploid cells with cell-cycle indicators were irradiated using a synchrotron X-ray microbeam, and mitochondrial membrane potential was quantified by JC-1 over the 72-h period postirradiation. Cytoplasmic irradiation was observed to temporarily enlarge the mitochondria! area with high membrane potential, while the total mitochondrial area did not change significantly. Unexpectedly, cell-nucleus irradiation promoted a similar increase not only in the mitochondria! areas with high membrane potential, but also in those with low membrane potential, which gave rise to the apparent increase in the total mitochondrial area. Augmentation of the mitochondrial area with low membrane potential was predominantly observed among G, cells, suggesting that nucleus irradiation during the G, phase regulated the mitochondrial dynamics of the cytoplasm, presumably through DNA damage in the nucleus. (C) 2020 by Radiation Research Society