High-speed solution switching using piezo-based micropositioning stages.
High-speed solution switching using piezo-based micropositioning stages.
复制标题
使用基于压电的微定位平台进行高速解决方案切换。
DOI：
10.1109/10.930905
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Devasia,S
中科院分区：
文献类型：
--
作者：
Stilson,S;McClellan,A;Devasia,S
运动诱导振动是用于实现溶液切换的高速微定位平台的关键限制。受控的快速溶液转换被用来研究在离体膜片中分离的配体门控离子通道群体的快速激活和失活动力学，这种研究是了解中枢神经系统中介导突触诱导和抑制的基本机制所必需的。然而，随着溶液切换速度的增加，在基于压电的定位平台中引起的振动可能导致不期望的、重复的配体施加到切除的贴片。本文介绍了一种方法，使用知识的压电工作台的振动动力学，以补偿和减少这些不必要的振动。该方法进行了实验验证，使用一个开放的电极技术，并实现快速的解决方案切换（100 /spl mu/s范围）。
Motion-induced vibration is a critical limitation in high-speed micropositioning stages used to achieve solution switching. Controlled rapid solution switching is used to study the fast activation and deactivation kinetics of ligand-gated ion-channel populations isolated in excised membrane patches-such studies are needed to understand fundamental mechanisms that mediate synaptic elicitation and inhibition in the central nervous system. However, as the solution-switching speed is increased, vibration induced in the piezo-based positioning stages can result in undesired, repeated, ligand application to the excised patch. The article describes a method to use knowledge of the piezo-stage's vibrational dynamics to compensate for and reduce these unwanted vibrations. The method was experimentally verified using an open-electrode technique, and fast solution switching (100 /spl mu/s range) was achieved.