Rice endophytic communities are strongly dependent on microbial communities specific to each soil
Rice endophytic communities are strongly dependent on microbial communities specific to each soil
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DOI：
10.1007/s00374-023-01743-3
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发表时间：
2023-05
影响因子：
6.5
通讯作者：
Solomon Oloruntoba Samuel;Kazuki Suzuki;Rasit Asiloglu;N. Harada
中科院分区：
文献类型：
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作者：
Solomon Oloruntoba Samuel;Kazuki Suzuki;Rasit Asiloglu;N. Harada
本研究旨在探讨土壤作为微生物来源对水稻根部内生细菌群落组成的影响。水稻秧苗采用水培方法，外加一个可渗透的袋子，里面填满了从日本不同地理位置收集的五种土壤类型中的一种。3周和6周后，采用基于Illumina MiSeq的16SrRNA基因扩增片段测序方法对水稻根部内生细菌群落进行分析。结果表明，作为微生物源添加的土壤中的细菌群落因土壤类型的不同而不同，这影响了水培溶液中的细菌群落，从而反映在内生细菌群落的组合中。细菌的多样性和丰富度因微生物来源的不同而有显著差异。因此，一个由伯克霍尔德氏菌-卡巴列尼亚-副伯克霍尔德氏菌组成的属水平上保守的由16个内生细菌组成的类群，与土壤类型无关，在所有微生物来源中共享，从而突显了水稻有选择地招募内生寄主的能力。总之，这项研究证明了微生物来源作为水稻根部内生细菌群落形成的关键驱动力的重要性。
This study aims to investigate the effects of soil as a microbial source on the assemblage of the endophytic bacterial communities in rice roots. Rice seedlings were grown hydroponically with the addition of a permeable bag filled with one of five soil types collected from different geographical locations in Japan. After 3 and 6 weeks, the endophytic bacterial communities in rice roots were analyzed using the Illumina Miseq-based 16SrRNA gene amplicon sequencing method. The results showed that the bacterial community in the soils added as a microbial source differed among the soil types, which affected the bacterial community in the hydroponic solution and consequently reflected in the endophytic bacterial community assemblage. Bacterial diversity and richness differed significantly with respect to the microbial sources. As a result, a conserved group of 16 endophytic bacterial taxa at the genus level, dominated byBurkholderia-Caballeronia-Paraburkholderia, and independent of the soil type were shared across all microbial sources, thereby underlining the ability of rice plants to selectively recruit their endophytic inhabitants. Altogether, this study demonstrates the importance of the microbial source as a crucial driving force for the formation of the endophytic bacterial communities in rice roots.