Kinetochore-localized BUB-1/BUB-3 complex promotes anaphase onset in C. elegans
Kinetochore-localized BUB-1/BUB-3 complex promotes anaphase onset in C. elegans
复制标题
DOI：
10.1083/jcb.201412035
复制
发表时间：
2015-05-25
影响因子：
7.8
通讯作者：
Desai, Arshad
中科院分区：
文献类型：
--
作者：
Kim, Taekyung;Moyle, Mark W.;Desai, Arshad
保守的 Bub1/Bub3 复合体被招募到有丝分裂染色体的动粒区域，在那里启动纺锤体检查点信号传导并促进染色体排列。在这里，我们表明，与检查点通路成分的预期相反，BUB-1/BUB-3 复合物促进秀丽隐杆线虫胚胎及时的后期开始。 BUB-1/BUB-3 的这种活性独立于纺锤体检查点信号传导，但需要动粒定位。 BUB-1/BUB-3 抑制等效地延迟了分离酶激活和有丝分裂退出期间发生的其他事件。后期促进功能需要BUB-1的激酶结构域，但不需要其激酶活性，并且该功能独立于BUB-1/BUB-3在染色体排列中的作用。这些结果揭示了 BUB-1/BUB-3 复合物在促进后期开始方面的意想不到的作用，这不同于其在检查点信号传导和染色体排列中经过充分研究的功能，并提出了一种有助于协调中期到后期转变的新机制。
The conserved Bub1/Bub3 complex is recruited to the kinetochore region of mitotic chromosomes, where it initiates spindle checkpoint signaling and promotes chromosome alignment. Here we show that, in contrast to the expectation for a checkpoint pathway component, the BUB-1/BUB-3 complex promotes timely anaphase onset in Caenorhabditis elegans embryos. This activity of BUB-1/BUB-3 was independent of spindle checkpoint signaling but required kinetochore localization. BUB-1/BUB-3 inhibition equivalently delayed separase activation and other events occurring during mitotic exit. The anaphase promotion function required BUB-1's kinase domain, but not its kinase activity, and this function was independent of the role of BUB-1/BUB-3 in chromosome alignment. These results reveal an unexpected role for the BUB-1/BUB-3 complex in promoting anaphase onset that is distinct from its well-studied functions in checkpoint signaling and chromosome alignment, and suggest a new mechanism contributing to the coordination of the metaphase-to-anaphase transition.