Effects of recombinant human endostatin on the expression of vascular endothelial growth factor in human gastric cancer cell line MGC-803
Effects of recombinant human endostatin on the expression of vascular endothelial growth factor in human gastric cancer cell line MGC-803
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DOI：
10.3892/br.2012.3
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发表时间：
2013-01-01
影响因子：
2.3
通讯作者：
Song, Zong-Min
中科院分区：
文献类型：
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作者：
Wang, Yu-Bin;Liu, Jun-Hong;Song, Zong-Min
本研究旨在探讨重组人内皮抑素恩度（ES）对人胃癌细胞系MGC-803中血管内皮生长因子（VEGF）表达的影响。采用免疫组织化学方法检测MGC-803细胞中VEGF蛋白的表达。用不同浓度的ES处理后，测定MGC-803细胞中mRNA和VEGF蛋白的表达。 MGC-803 细胞中检测到高水平的 VEGF 蛋白表达。 ES处理后，MGC-803细胞中mRNA和VEGF蛋白表达显着降低（所有P
The present study aimed to explore the effects of recombinant human endostatin, endostar (ES), on the expression of vascular endothelial growth factor (VEGF) in the human gastric cancer cell line MGC-803. The expression of VEGF protein in MGC-803 cells was examined using immunohistochemistry. Subsequent to treatment with various concentrations of ES, the mRNA and VEGF protein expressions were determined in MGC-803 cells. A high level of VEGF protein expression was detected in MGC-803 cells. Subsequent to ES treatment, the mRNA and VEGF protein expressions were significantly decreased in MGC-803 cells (all P