NOD-like receptors in the human upper airways: a potential role in nasal polyposis
NOD-like receptors in the human upper airways: a potential role in nasal polyposis
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DOI：
10.1111/j.1398-9995.2010.02527.x
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发表时间：
2011-05-01
期刊：
影响因子：
12.4
通讯作者：
Cardell, L. O.
中科院分区：
文献类型：
--
作者：
Mansson, A.;Bogefors, J.;Cardell, L. O.
P>背景：核苷酸结合寡聚结构域（NOD）样受体（NLR）是新发现的胞质受体，属于模式识别受体家族。它们检测各种与病原体相关的分子模式，从而引发免疫反应。关于这些受体及其在健康和疾病中的作用的知识是有限的。本研究的目的是表征人类上呼吸道中 NOD1、NOD2 和 NALP3 的表达。方法：从扁桃体疾病 (n = 151)、腺样体肥大 (n = 9) 和鼻息肉病 (n = 24) 患者中获取手术样本。鼻活检取自健康志愿者 (n = 10)。采用实时荧光定量PCR和免疫组化方法分析NOD1、NOD2和NALP3的表达。结果：所有组织标本中均可见NOD1、NOD2和NALP3 mRNA和蛋白的表达。研究发现鼻息肉中的 NLR mRNA 高于正常鼻粘膜，局部类固醇治疗可降低息肉中 NLR 的表达。相比之下，扁桃体感染化脓性链球菌或流感嗜血杆菌并不影响NLR的表达。结论：本研究证明了NLR在几种上呼吸道组织中的存在，并强调了NLR在伴有息肉的慢性鼻窦炎中的潜在作用。
P>Background:Nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs) are newly discovered cytosolic receptors belonging to the pattern-recognition receptor family. They detect various pathogen-associated molecular patterns, triggering an immune response. The knowledge about these receptors, and their role in health and disease, is limited. The aim of the present study was to characterize the expression of NOD1, NOD2, and NALP3 in the human upper airways.Methods:Surgical samples were obtained from patients with tonsillar disease (n = 151), hypertrophic adenoids (n = 9), and nasal polyposis (n = 24). Nasal biopsies were obtained from healthy volunteers (n = 10). The expression of NOD1, NOD2, and NALP3 was analyzed using real-time PCR and immunohistochemistry.Results:Expression of NOD1, NOD2, and NALP3 mRNA and protein were seen in all tissue specimens. The NLR mRNA was found to be higher in nasal polyps than in normal nasal mucosa, and local steroid treatment reduced the NLR expression in polyps. In contrast, tonsillar infection with Streptococcus pyogenes or Haemophilus influenzae did not affect the NLR expression.Conclusions:The present study demonstrates the presence of NLRs in several upper airway tissues and highlights a potential role of NLRs in chronic rhinosinusitis with polyps.