Nongenomic effects of progesterone on spermatozoa: mechanisms of signal transduction and clinical implications.
Nongenomic effects of progesterone on spermatozoa: mechanisms of signal transduction and clinical implications.
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DOI：
10.1080/15513819809168798
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发表时间：
1998-07
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影响因子：
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通讯作者：
Elisabetta Baldi;M. Luconi;L. Bonaccorsi;Gianni Forti
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文献类型：
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作者：
Elisabetta Baldi;M. Luconi;L. Bonaccorsi;Gianni Forti
孕酮（P）是人类精子顶体反应的生理刺激物之一。它在受精部位（卵丘）以高水平存在，并被描述为影响几种精子功能，包括运动力，获能和顶体反应。类固醇在卵母细胞周围的卵丘基质中以高水平存在，其作用由细胞内钙浓度增加、氯离子流出、磷脂酶活性刺激和蛋白质磷酸化介导。这些效应是由于快速/非基因组途径的激活。最近在人类精子表面发现了两种不同类型的P受体，与基因组受体不同。P对这些受体的亲和力分别在纳米和微摩尔范围内。精子对孕酮的反应性在生育力低下的患者中受损，并且与卵母细胞的受精能力严格相关。此外，精子反应性的测定可预测可交配性，阳性预测值为90%，可用于临床初步评估男性伴侣，以选择适当的辅助生殖技术。
Progesterone (P) is one of the physiological stimuli of human sperm acrosome reaction. It is present in high levels at the site of fertilization (cumulus oophorus) and has been describe to affect several sperm functions including motility, capacitation and acrosome reaction. The effects of the steroid, which is present in high levels in the cumulus matrix that surrounds the oocyte, are mediated by an increase of intracellular calcium concentrations, efflux of chloride, stimulation of activity of phospholipases and phosphorylation of proteins. These effects are due to activation of a rapid/nongenomic pathway. Two different types of receptors for P, distinct from the genomic ones, have been recently identified on the surface of human spermatozoa. The affinities of P for these receptors are respectively in the nano- and in the micromolar range. Sperm responsiveness to progesterone is impaired in subfertile patients and is strictly correlated to the ability of fertilize the oocyte. In addition, the determination of sperm responsiveness is predictive of fertilizing ability with a positive predictive value of 90% and can be clinically useful for the preliminary assessment of the male partner to select the appropriate assisted reproductive technique.