Effects of various culture environments on expression of major outer membrane proteins from Porphyromonas gingivalis.
Effects of various culture environments on expression of major outer membrane proteins from Porphyromonas gingivalis.
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DOI：
10.1016/s0378-1097(03)00896-6
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发表时间：
2004
影响因子：
2.1
通讯作者：
Y. Murakami;Masashi Imai;Y. Mukai;S. Ichihara;Hiroshi Nakamura;F. Yoshimura
中科院分区：
文献类型：
--
作者：
Y. Murakami;Masashi Imai;Y. Mukai;S. Ichihara;Hiroshi Nakamura;F. Yoshimura
我们研究了各种培养环境对牙龈卟啉单胞菌ATCC 33277主要外膜蛋白的影响。主要的外膜蛋白的凝胶电泳模式显示，在可培养的渗透压和pH值范围内的差异不大。随着温度升高或培养时间的延长，牙龈卟啉菌蛋白酶带的强度下降，但是，带的RagA，RagB和推定的孔蛋白是相对稳定的。用几种不同的培养基观察到类似的结果。尽管RagA、RagB和假定的孔蛋白的确切功能尚不清楚，但这些因素可能与成人牙周炎的发生和发展密切相关。
We examined the effects of various culture environments on major outer membrane proteins fromPorphyromonas gingivalisATCC 33277. Major outer membrane protein patterns on gel electrophoresis showed little difference over the culturable range of osmolarity and pH. With elevated temperature or prolonged culture, the intensities of the gingipain bands decreased; however, bands of RagA, RagB and the putative porins were relatively stable. Similar results were observed with several different culture media. Although the precise functions of RagA, RagB and the putative porins are unknown, these factors may be strongly related to the initiation and progression of adult periodontitis.