Growth of desferrioxamine-deficient Streptomyces mutants through xenosiderophore piracy of airborne fungal contaminations
Growth of desferrioxamine-deficient Streptomyces mutants through xenosiderophore piracy of airborne fungal contaminations
复制标题
DOI：
10.1093/femsec/fiv080
复制
发表时间：
2015-07-01
影响因子：
4.2
通讯作者：
Rigali, Sebastien
中科院分区：
文献类型：
--
作者：
Argueelles Arias, Anthony;Lambert, Stephany;Rigali, Sebastien
由于铁对管家功能、营养、形态发生和次生代谢物的产生是必需的，铁载体盗用可能是微生物在土壤和底物定植中的关键策略。在这里，我们报告了不能产生去铁胺铁载体的天蓝色链霉菌突变株，当培养皿被一种青霉菌和恩乔登酸菌的室内空气孢子污染时，可以恢复生长。UPLC-ESI-MS分析表明，含有青霉菌胞外复苏因子的高效液相色谱组分只含有铁载体，即铁二聚体酸、铁色素、褐藻碱C和辅原。缺乏去铁胺的链霉菌突变株的恢复生长很可能是通过摄取外源铁载体介导的，因为培养能力取决于编码ABC转运蛋白相关的铁载体结合蛋白的基因。丝状真菌允许去铁胺非产生链霉菌在共培养中生长，证实了外源铁载体海盗在这些分类上不相关的丝状微生物之间的营养相互作用中发挥了至关重要的作用。
Due to the necessity of iron for housekeeping functions, nutrition, morphogenesis and secondary metabolite production, siderophore piracy could be a key strategy in soil and substrate colonization by microorganisms. Here we report that mutants of bacterium Streptomyces coelicolor unable to produce desferrioxamine siderophores could recover growth when the plates were contaminated by indoor air spores of a Penicillium species and Engyodontium album. UPLC-ESI-MS analysis revealed that the HPLC fractions with the extracellular 'resuscitation' factors of the Penicillium isolate were only those that contained siderophores, i.e. Fe-dimerum acid, ferrichrome, fusarinine C and coprogen. The restored growth of the Streptomyces mutants devoid of desferrioxamine is most likely mediated through xenosiderophore uptake as the cultivability depends on the gene encoding the ABC-transporter-associated DesE siderophore-binding protein. That a filamentous fungus allows the growth of desferrioxamine non-producing Streptomyces in cocultures confirms that xenosiderophore piracy plays a vital role in nutritional interactions between these taxonomically unrelated filamentous microorganisms.