Characterization and molecular analysis of macrolide-resistant Mycoplasma pneumoniae clinical isolates obtained in Japan
Characterization and molecular analysis of macrolide-resistant Mycoplasma pneumoniae clinical isolates obtained in Japan
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DOI：
10.1128/aac.48.12.4624-4630.2004
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发表时间：
2004-12-01
影响因子：
4.9
通讯作者：
Sasaki, T
中科院分区：
文献类型：
--
作者：
Matsuoka, M;Narita, M;Sasaki, T
近年来，在日本偶尔会遇到对大环内酯类抗生素具有临床耐药性的肺炎支原体菌株。在2000年至2003年期间从日本三个不同地区分离出的76株肺炎支原体中，有13株对红霉素（ERY）耐药。在这13株中，12株对ERY高度耐药（最低抑菌浓度≥256微克/毫升），1株为轻度耐药（最低抑菌浓度为8微克/毫升）。对与ERY耐药性相关的23S rRNA的H区和V区以及核糖体蛋白L4和L22进行核苷酸测序表明，10株在2063位（对应大肠杆菌编号的2058位）发生了A - G转换，1株在2063位发生了A - C颠换，1株在2064位发生了A - G转换，轻度ERY耐药株在V区的2617位（对应大肠杆菌编号的2611位）发生了C - G颠换。第11区以及核糖体蛋白L4和L22与这些临床肺炎支原体菌株的ERY耐药性无关。此外，利用我们建立的限制性片段长度多态性技术检测肺炎支原体23S rRNA基因V区PCR产物的点突变，我们发现从呼吸道感染儿童采集的94份PCR阳性口腔样本中有23份（24%）出现了A2063G突变。这些结果表明，在日本，ERY耐药性肺炎支原体感染并不罕见。
In recent years, Mycoplasma pneumoniae strains that are clinically resistant to macrolide antibiotics have occasionally been encountered in Japan. Of 76 strains of M. pneumoniae isolated in three different areas in Japan during 2000 to 2003, 13 strains were erythromycin (ERY) resistant. Of these 13 strains, 12 were highly ERY resistant (MIC, greater than or equal to256 mug/ml) and 1 was weakly resistant (MIC, 8 mug/ml). Nucleotide sequencing of domains H and V of 23S rRNA and ribosomal proteins L4 and L22, which are associated with ERY resistance, showed that 10 strains had an A-to-G transition at position 2063 (corresponding to 2058 in Escherichia coli numbering), 1 strain showed A-to-C transversion at position 2063, 1 strain showed an A-to-G transition at position 2064, and the weakly ERY-resistant strain showed C-to-G transversion at position 2617 (corresponding to 2611 in E. coli numbering) of domain V. Domain 11 and ribosomal proteins L4 and L22 were not involved in the ERY resistance of these clinical M. pneumoniae strains. In addition, by using our established restriction fragment length polymorphism technique to detect point mutations of PCR products for domain V of the 23S rRNA gene of M. pneumoniae, we found that 23 (24%) of 94 PCR-positive oral samples taken from children with respiratory infections showed A2063G mutation. These results suggest that ERY-resistant M. pneumoniae infection is not unusual in Japan.