Presence of polysialic acid and HNK-1 carbohydrate on brain glycoproteins from β-l,4-galactosyltransferase-knockout mice
Presence of polysialic acid and HNK-1 carbohydrate on brain glycoproteins from β-l,4-galactosyltransferase-knockout mice
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DOI：
10.1006/bbrc.1998.8537
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发表时间：
1998-04-28
影响因子：
3.1
通讯作者：
Furukawa, K
中科院分区：
文献类型：
--
作者：
Kido, M;Asano, M;Furukawa, K
多唾液酸和HNK-1碳水化合物在特定发育阶段表达于神经细胞识别分子n链糖链的Gal β 1—bbb4glcnac外链上，参与神经组织的形成。小鼠β -1,4-半乳糖转移酶（β -1,4- galt）基因的靶向失活导致小鼠寿命缩短，这些小鼠被认为没有这种碳水化合物抗原，但在神经组织形成方面没有缺陷。对突变小鼠脑糖蛋白的分析显示，聚唾液酸和HNK-1碳水化合物通常以年龄依赖的方式表达。为了支持这一观点，蛋白质带与蓖ricinus communis凝集素- i反应，后者与以Gal β 1- >4GlcNAc组结尾的寡糖相互作用，并且在突变小鼠大脑中检测到β -1,4- galt对GlcNAc β - s - pnp的活性，表明大脑中含有另一种对碳水化合物抗原表达重要的功能性β -1,4- galt。(C) 1998学术出版社。
Polysialic acid and HNK-1 carbohydrate are expressed on Gal beta 1-->4GlcNAc outer chains of N-linked sugar chains of neural cell recognition molecules at certain developmental stages and involved in neural tissue formation. Targeted inactivation of the mouse beta-1,4-galactosyltransferase (beta-1,4-GalT) gene resulted in short life of the mice which supposedly do not have such carbohydrate antigens but have no defects in neural tissue formation. Analysis of the mutant mouse brain glycoproteins revealed that polysialic acid and HNK-1 carbohydrate are normally expressed in an age-dependent manner. In support of this, protein bands reacted with Ricinus communis agglutinin-I, which interacts with oligosaccharides terminated with the Gal beta 1-->4GlcNAc group, and beta-1,4-GalT activity toward GlcNAc beta-S-pNP were detected in the mutant mouse brain, indicating that brain contains another functional beta-1,4-GalT important for the expression of the carbohydrate antigens. (C) 1998 Academic Press.