Organ-specific expression of the divalent ion channel proteins NCKX3, TRPV2, CTR1, ATP7A, IREG1 and HEPH in various canine organs
Organ-specific expression of the divalent ion channel proteins NCKX3, TRPV2, CTR1, ATP7A, IREG1 and HEPH in various canine organs
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DOI：
10.3892/mmr.2018.9148
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发表时间：
2018-08-01
影响因子：
3.4
通讯作者：
Jeung, Eui-Bae
中科院分区：
文献类型：
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作者：
Ahn, Changhwan;Choi, Jong-Sam;Jeung, Eui-Bae
跨膜阳离子通道包括用于钙、铜和铁离子运输的通道。每个通道都具有生理意义，并且都与疾病相关。然而，此前尚未报道过犬器官转录翻译水平的比较研究。在本研究中，钙通道，包括钠/钾/钙交换器3（NCKX3）和瞬时受体电位阳离子通道亚家族V成员2（TRPV2），铜通道，包括高亲和力铜摄取蛋白1（CTR1）和铜转运ATP酶1（ATP7A），以及铁通道，包括铁调节转运蛋白1（IREG1）和铁黄蛋白（HEPH）蛋白及其mRNA的器官特异性表达被检查犬的十二指肠、肾脏、脾脏和肝脏。 NCKX3蛋白在肾脏中表达量最高，十二指肠中表达量中等，脾脏和肝脏中最低；TRPV2蛋白在肾脏、十二指肠和肝脏中表达量较高，脾脏中表达量较低。 CTR1蛋白表达水平在肝脏中最高，其次（按降序排列）是十二指肠、肾脏和脾脏。 ATP7A蛋白表达水平在十二指肠中最高，在脾中最低。 IREG1蛋白表达水平在肝脏中最高，其次（按降序排列）是肾脏、十二指肠和脾脏。 HEPH蛋白水平在肝脏中较高，在十二指肠和肾脏中中等，在脾中较低。免疫组织化学分析的结果证明了离子通道蛋白的定位。这些结果表明，阳离子通道蛋白在犬器官之间存在差异表达，并且它们可能参与与维持生理稳态相关的器官特异性功能。
Transmembrane cation channels include those for calcium, copper and iron ion transport. Each channel has physiological significance, and all have been associated with disease. However, the comparative study of transcriptional-translational levels in canine organs has not been previously reported. In the present study, organ-specific expression of calcium channels, including sodium/potassium/calcium exchanger 3 (NCKX3) and transient receptor potential cation channel subfamily V member 2 (TRPV2), copper channels, including high affinity copper uptake protein 1 (CTR1) and copper-transporting ATPase 1 (ATP7A), and iron channels, including iron-regulated transporter 1 (IREG1) and hephaestin (HEPH) proteins and their mRNAs were examined in the canine duodenum, kidney, spleen and liver. NCKX3 protein expression was highest in the kidney, moderate in the duodenum, and lowest in the spleen and liver, whereas TRPV2 protein was highly expressed in the kidney, duodenum and liver, and was low in the spleen. The CTR1 protein expression level was highest in the liver, followed (in descending order) by the duodenum, kidney and spleen. The ATP7A protein expression level was highest in the duodenum and lowest in the spleen. The IREG1 protein expression level was highest in the liver, followed (in descending order) by the kidney, duodenum and spleen. The HEPH protein level was high in liver, moderate in the duodenum and kidney, and low in the spleen. The results of the immunohistochemistry analysis demonstrated ion channel protein localizations. These results suggested that cation channel proteins are differentially expressed among canine organs, and they may be involved in organ-specific functions associated with the maintenance of physiological homeostasis.