Reparative properties of the traditional Chinese medicine Cordyceps sinensis (Chinese caterpillar mushroom) using HT29 cell culture and rat gastric damage models of injury
Reparative properties of the traditional Chinese medicine Cordyceps sinensis (Chinese caterpillar mushroom) using HT29 cell culture and rat gastric damage models of injury
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DOI：
10.1017/s0007114510005118
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发表时间：
2011-05-01
影响因子：
3.6
通讯作者：
Playford, Raymond J.
中科院分区：
文献类型：
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作者：
Marchbank, Tania;Ojobo, Ehighale;Playford, Raymond J.
冬虫夏草(CS)是一种中药和保健食品，用于支持多种器官系统。它的商业生产是通过在液体介质中或在固体(谷物/土豆)相中培养来生产的。我们测试了商用CS的热水提取液和固相水提物对人结肠癌HT29细胞增殖(氧化还原指示剂阿拉马蓝)、迁移(连续损伤单层光学显微镜)、胶原凝胶侵袭(荧光法)和吲哚美辛诱导的人结肠癌HT29细胞凋亡(活性caspase-3比色法)的影响。体内实验采用大鼠胃损伤模型(消炎痛20 mg/kg，灌胃束缚4h)。当浓度为10 mg/ml时，CS提取物可刺激细胞增殖3倍(P
Cordyceps sinensis (CS) is a traditional Chinese medicine and health food used to support many organ systems. It is commercially produced by cultivation in a liquid medium or on a solid (grain/potato) phase. We tested the effects of hot water extracts of liquid-phase and solid-phase commercially grown CS on its ability to influence proliferation (using Alamar blue, an oxidation/reduction indicator), migration (serial-wounded monolayer photomicroscopy), invasion through collagen gel (fluorometric assay) and indomethacin-induced apoptosis (active caspase-3 colorimetric assay) of human colon cancer HT29 cells. An in vivo study used a rat gastric damage model (indomethacin 20 mg/kg and 4 h restraint with oral administration). The CS extract stimulated cell proliferation threefold when added at 10 mg/ml (P