Cloning and expression analysis of three novel CC chemokine genes from Japanese flounder (Paralichthys olivaceus).
Cloning and expression analysis of three novel CC chemokine genes from Japanese flounder (Paralichthys olivaceus).
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DOI：
10.1016/j.fsi.2014.08.003
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发表时间：
2014-10
影响因子：
4.7
通讯作者：
Gang Zou;R. Nozaki;H. Kondo;I. Hirono
中科院分区：
文献类型：
--
作者：
Gang Zou;R. Nozaki;H. Kondo;I. Hirono
趋化因子是由各种细胞类型分泌的小细胞因子。它们不仅在细胞活化、分化和运输中起作用，而且还对许多生物学过程产生影响。本研究从日本比目鱼中克隆了三个新的CC趋化因子基因Paol-SCYA 105、106和107，Paol-SCYA 105主要在鳃、肾脏和脾脏中检测到，Paol-SCYA 106在所有检测的组织中检测到，Paol-SCYA 107主要在脾和肾中检测到，Paol-SCYA 105和Paol-SCYA 107主要在脾和肾中检测到。病毒性出血性败血症病毒感染后第3天，SCYA 106基因表达达到高峰，第6天开始下降，Paol-SCYA 106基因在第6天仍维持在较高水平;病毒性出血性败血症病毒感染后第6天，Paol-SCYA 107基因在肾脏表达显著上调。在革兰氏阴性迟缓爱德华氏菌和革兰氏阳性海豚链球菌感染的肾脏中，只有Paol-SCYA 106基因表达显著增加。总之，这些结果表明，这三种新型CC趋化因子参与了针对病原体感染的免疫应答。
Chemokines are small cytokines secreted by various cell types. They not only function in cell activation, differentiation and trafficking, but they also have influences on many biological processes. In this study, three novel CC chemokine genesPaol-SCYA105, 106 and 107 in Japanese flounder (Paralichthys olivaceus) were cloned and characterized.Paol-SCYA105 was mainly detected in gill, kidney and spleen,Paol-SCYA106 was detected in all tissues examined andPaol-SCYA107 was mainly detected in the spleen and kidney.Paol-SCYA105 andPaol-SCYA106 gene expressions peaked in kidney at day 3 after viral hemorrhagic septicemia virus infection and decreased at day 6, butPaol-SCYA106 still remained at a high level at day 6.Paol-SCYA107 gene expression was significantly up-regulated in kidney at day 6 after viral hemorrhagic septicemia virus infection. In response to infection by Gram-negative Edwardsiella tarda and Gram-positive Streptococcus iniae in kidney, onlyPaol-SCYA106 gene expression significantly increased. Together, these results indicate that these three novel CC chemokines are involved in the immune response against pathogen infections.