Organization and expression of mouse nm23-M1 gene. Comparison with nm23-M2 expression.
Organization and expression of mouse nm23-M1 gene. Comparison with nm23-M2 expression.
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小鼠 nm23-M1 基因的组织和表达。
DOI：
10.1016/s0378-1119(99)00288-7
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发表时间：
1999
期刊：
影响因子：
3.5
通讯作者：
J. Daniel
中科院分区：
文献类型：
--
作者：
S. Dabernat;M. Larou;K. Massé;E. Dobremez;M. Landry;C. Mathieu;J. Daniel
Nm 23是编码核苷酸二磷酸激酶（NDPK）的不同亚型的基因家族，NDPK是参与核苷三磷酸合成的酶。在本研究中，nm 23-M1基因编码的小鼠NDPKA亚型的组织和表达进行了描述。该基因长约10 kb，由5个外显子组成。与人类和大鼠相关基因相比，组织和外显子-内含子边界严格保守。该基因启动子区没有显示任何共有TATA盒、SP1结合元件或Inr序列。相比之下，TCF-1/LEF-1结合元件和Pit-1共有序列存在。用北方杂交和原位杂交方法分别对成年和孕18.5天小鼠胚胎进行了研究。他们表现出组织特异性表达nm 23-M1转录本，尽管管家基因启动子功能。在神经系统、感觉器官和胚胎胸腺中检测到最强的信号。相反，nm 23-M2 mRNA显示更广泛地表达。对nm 23-M1基因组织特异性表达与启动子区结合元件的关系进行了讨论。
Nm23 is a gene family encoding different isoforms of the nucleotide diphosphate kinase (NDPK), an enzyme involved in the synthesis of nucleoside triphosphates. In the present study, the organization and expression of the nm23-M1 gene encoding the mouse NDPKA isoform are described. This gene is about 10kb long and composed of five exons. The organization and the exon–intron boundaries are strictly conserved as compared to the human and rat related genes. The gene promoter region did not exhibit any consensus TATA box, SP1 binding element or Inr sequence. By contrast, TCF-1/LEF-1 binding elements and Pit-1 consensus sequence were present. Northern blotting and in situ hybridization methods were carried out in adult and 18.5days post-coitum (dpc) mouse embryo, respectively. They showed tissue-specific expression of nm23-M1 transcripts, despite housekeeping gene promoter features. The strongest signals were detected in the nervous system, sensory organs and embryonic thymus. In contrast nm23-M2 mRNA was shown to be more widely expressed. The relationship between nm23-M1 gene tissue-specific expression and the putative binding element of the promoter region is discussed.
DOI：
10.1016/s0021-9258(17)37011-4
发表时间：
1994-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
E. Postel;C. Ferrone
通讯作者：
E. Postel;C. Ferrone