In Vivo Bioluminescence Imaging of Intratumoral Bacteria
In Vivo Bioluminescence Imaging of Intratumoral Bacteria
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DOI：
10.1007/978-1-4939-3515-4_7
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发表时间：
2016-01-01
期刊：
影响因子：
--
通讯作者：
Tangney, Mark
中科院分区：
文献类型：
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作者：
Cronin, Michelle;Akin, Ali R.;Tangney, Mark
本章介绍了使用全身生物发光成像（BLI）的研究细菌贩运活小鼠，重点是使用细菌治疗癌症。细菌是一类有吸引力的癌症治疗载体，具有在全身给药后优先在肿瘤内生长的天然能力。经工程改造以表达lux基因盒的细菌允许BLI同时检测细菌和肿瘤部位。随着时间的推移，肿瘤内细菌的位置和水平可以很容易地检查，在二维或三维可视化。该方法适用于广泛的细菌物种和肿瘤异种移植物类型。本文描述了分析皮下肿瘤小鼠体内的生物发光细菌的协议。这种功能强大、价格低廉的实时成像策略是在癌症研究背景下研究体内细菌的理想方法。该方案概述了在小鼠中研究lux标记的大肠杆菌和短双歧杆菌的程序，证明了全身体内发光成像可实现的2D和3D BLI的空间和时间读数。
This chapter describes the use of whole-body bioluminescent imaging (BLI) for the study of bacterial traffi cking in live mice, with an emphasis on the use of bacteria in therapy of cancer. Bacteria present an attractive class of vector for cancer therapy, possessing a natural ability to grow preferentially within tumors following systemic administration. Bacteria engineered to express the lux gene cassette permit BLI detection of the bacteria and tumor sites concurrently. The location and levels of bacteria within tumors over time can be readily examined, visualized in two or three dimensions. The method is applicable to a wide range of bacterial species and tumor xenograft types. This article describes the protocol for analysis of bioluminescent bacteria within subcutaneous tumor-bearing mice. This powerful, and inexpensive, realtime imaging strategy represents an ideal method for the study of bacteria in vivo in the context of cancer research. This protocol outlines the procedure for studying lux-tagged Escherichia coli and Bifi dobacterium breve in mice, demonstrating the spatial and temporal readout from 2D and 3D BLI achievable with whole-body in vivo luminescence imaging.